Recombinant Mouse IL-1ra/IL-1F3 Protein

Formulations:
Catalog # Availability Size / Price Qty
480-RM-010
480-RM-050
Product Details
Citations (23)
FAQs
Reviews (3)

Recombinant Mouse IL-1ra/IL-1F3 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 15‑60 ng/mL in the presence of 50 pg/mL of recombinant human IL-1 alpha.
Source
E. coli-derived mouse IL-1ra/IL-1F3 protein
Arg8-Gln159, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
17 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

480-RM

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

480-RM/CF

Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid, NaCl and EDTA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally-occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells. In mouse, IL-1 RII does not bind IL-1ra. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Pre-clinical and clinical studies were done to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Long Name
Interleukin 1 Receptor Antagonist
Entrez Gene IDs
3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)
Alternate Names
DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

Citations for Recombinant Mouse IL-1ra/IL-1F3 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Pathological pain processing in mouse models of multiple sclerosis and spinal cord injury: contribution of plasma membrane calcium ATPase 2 (PMCA2)
    Authors: E Mirabelli, L Ni, L Li, C Acioglu, RF Heary, S Elkabes
    J Neuroinflammation, 2019;16(1):207.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Dengue Virus Infection Activates Interleukin-1beta to Induce Tissue Injury and Vascular Leakage
    Authors: P Pan, Q Zhang, W Liu, W Wang, Z Yu, Z Lao, W Zhang, M Shen, P Wan, F Xiao, MA Shereen, W Zhang, Q Tan, Y Liu, X Liu, K Wu, Y Liu, G Li, J Wu
    Front Microbiol, 2019;10(0):2637.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. Pathogenic function of bystander-activated memory-like CD4+ T cells in autoimmune encephalomyelitis
    Authors: HG Lee, JU Lee, DH Kim, S Lim, I Kang, JM Choi
    Nat Commun, 2019;10(1):709.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. AMPK activation attenuates inflammatory pain through inhibiting NF-?B activation and IL-1? expression
    Authors: HC Xiang, LX Lin, XF Hu, H Zhu, HP Li, RY Zhang, L Hu, WT Liu, YL Zhao, Y Shu, HL Pan, M Li
    J Neuroinflammation, 2019;16(1):34.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  5. Telomere Dysfunction Disturbs Macrophage Mitochondrial Metabolism and the NLRP3 Inflammasome through the PGC-1?/TNFAIP3 Axis
    Authors: Y Kang, H Zhang, Y Zhao, Y Wang, W Wang, Y He, W Zhang, W Zhang, X Zhu, Y Zhou, L Zhang, Z Ju, L Shi
    Cell Rep, 2018;22(13):3493-3506.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. Gene deficiency and pharmacological inhibition of caspase-1 confers resilience to chronic social defeat stress via regulating the stability of surface AMPARs
    Authors: MX Li, HL Zheng, Y Luo, JG He, W Wang, J Han, L Zhang, X Wang, L Ni, HY Zhou, ZL Hu, PF Wu, Y Jin, LH Long, H Zhang, G Hu, JG Chen, F Wang
    Mol. Psychiatry, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  7. Interaction between interleukin-1? and type-1 cannabinoid receptor is involved in anxiety-like behavior in experimental autoimmune encephalomyelitis
    J Neuroinflammation, 2016;13(1):231.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. Deficiency of autophagy protein Map1-LC3b mediates IL-17-dependent lung pathology during respiratory viral infection via ER stress-associated IL-1.
    Authors: Reed M, Morris S, Owczarczyk A, Lukacs N
    Mucosal Immunol, 2015;8(5):1118-30.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions.
    Authors: Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S
    PLoS ONE, 2015;10(1):e0117908.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Inflammasome activation contributes to interleukin-23 production in response to Clostridium difficile.
    Authors: Cowardin C, Kuehne S, Buonomo E, Marie C, Minton N, Petri W
    MBio, 2015;6(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. The PYRIN domain-only protein POP3 inhibits ALR inflammasomes and regulates responses to infection with DNA viruses.
    Authors: Khare S, Ratsimandresy R, de Almeida L, Cuda C, Rellick S, Misharin A, Wallin M, Gangopadhyay A, Forte E, Gottwein E, Perlman H, Reed J, Greaves D, Dorfleutner A, Stehlik C
    Nat Immunol, 2014;15(4):343-53.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Age-Dependent Neuroimmune Modulation of IGF-1R in the Traumatic Mice.
    Authors: Zhao H, Zhao X, Cao X, Wu G
    Immun Ageing, 2012;9(1):12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Nuclear factor-kappaB is a critical mediator of stress-impaired neurogenesis and depressive behavior.
    Authors: Koo JW, Russo SJ, Ferguson D, Nestler EJ, Duman RS
    Proc. Natl. Acad. Sci. U.S.A., 2010;107(6):2669-74.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  14. Central inhibition of interleukin-1beta ameliorates sickness behavior in aged mice.
    Authors: Abraham J, Johnson RW
    Brain Behav. Immun., 2009;23(3):396-401.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  15. Stratification is the key: inflammatory biomarkers accurately direct immunomodulatory therapy in experimental sepsis.
    Authors: Osuchowski MF, Connett J, Welch K, Granger J, Remick DG
    Crit. Care Med., 2009;37(5):1567-73.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  16. Comparison of the roles of IL-1, IL-6, and TNFalpha in cell culture and murine models of aseptic loosening.
    Authors: Taki N, Tatro JM, Lowe R, Goldberg VM, Greenfield EM
    Bone, 2007;40(5):1276-83.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Interleukin 1 receptor antagonist mediates the antiinflammatory and antifibrotic effect of mesenchymal stem cells during lung injury.
    Authors: Ortiz LA, Dutreil M, Fattman C, Pandey AC, Torres G, Go K, Phinney DG
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(26):11002-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  19. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  20. Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
    Authors: Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S
    Neuroscience, 2005;132(3):625-31.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  21. IL-1 mediates TNF-induced osteoclastogenesis.
    Authors: Wei S, Kitaura H, Zhou P, Ross FP, Teitelbaum SL
    J. Clin. Invest., 2005;115(2):282-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Brain interleukin-1 is involved in spatial memory and passive avoidance conditioning.
    Authors: Yirmiya R, Winocur G, Goshen I
    Neurobiol Learn Mem, 2002;78(2):379-89.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  23. Alteration of locus coeruleus neuronal activity by interleukin-1 and the involvement of endogenous corticotropin-releasing hormone.
    Authors: Borsody MK, Weiss JM
    Neuroimmunomodulation, 2002;10(2):101-21.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Mouse IL-1ra/IL-1F3 Protein

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Recombinant Mouse IL-1ra/IL-1F3 Protein
By Anonymous on 12/22/2020
Application: In vitro bioactivity in cell culture

Recombinant Mouse IL-1ra/IL-1F3 Protein
By Anonymous on 09/30/2020
Application: Binding assay/Protein-protein interaction

Recombinant Mouse IL-1ra/IL-1F3 Protein
By Anonymous on 06/27/2018
Application: In vitro bioactivity in cell culture