Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4+ CD45RBlow memory T cells, but at much lower levels by mouse CD4+ CD45RBhigh cells. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.
Recombinant Mouse IL-23R Fc Chimera Protein, CF
R&D Systems | Catalog # 1686-MR
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Key Product Details
- R&D Systems NS0-derived Recombinant Mouse IL-23R Fc Chimera Protein (1686-MR)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Binding Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse IL-23 R protein
| Mouse IL-23 R (Gly24-Asp372) Accession # Q8K4B4 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Gly24
Predicted Molecular Mass
66.8 kDa (monomer)
SDS-PAGE
105 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Mouse IL-23 R/Fc Chimera is immobilized at 5 µg/mL (100 µL/well) the concentration of Recombinant Mouse IL‑23 (Catalog # 1887-ML) that produces 50% of optimal binding response is approximately 5-25 ng/mL
When Recombinant Mouse IL-23 R/Fc Chimera is immobilized at 5 µg/mL (100 µL/well) the concentration of Recombinant Mouse IL‑23 (Catalog # 1887-ML) that produces 50% of optimal binding response is approximately 5-25 ng/mL
Reviewed Applications
Read 1 review rated 5 using 1686-MR in the following applications:
Formulation, Preparation, and Storage
1686-MR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: IL-23R
References
- Oppmann, B. et al. (2000) Immunity 13:715.
- Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
- Parham, C. et al. (2002) J. Immunol. 168:5448.
- Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
- Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.
Long Name
Interleukin 23 Receptor
Alternate Names
IL-23 R, IL23R
Gene Symbol
IL23R
UniProt
Additional IL-23R Products
Product Documents for Recombinant Mouse IL-23R Fc Chimera Protein, CF
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse IL-23R Fc Chimera Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Mouse IL-23R Fc Chimera Protein, CF
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 01/08/2018BLI experiment to assess the binding of IL-23 and mutants to IL-23R. Recombinant Mouse IL-23 R Fc Chimera Protein was immobilized on anti-human IgG Fc capture biosensors. Sensograms and fit of 1:1 model of 3 fold dilution series of analyte starting at 517 nM. (Bloch et al. Immunity 2018 DOI: http://dx.doi.org/10.1016/j.immuni.2017.12.008)
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