Recombinant Mouse Noggin Fc Chimera Protein, CF

R&D Systems | Catalog # 719-NG

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Noggin Fc Chimera Protein (719-NG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

U79163

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all Noggin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Noggin protein
Mouse Noggin
(Leu20-Cys232)
Accession # U79163		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu20

Predicted Molecular Mass

50 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.06-0.3 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (Catalog # 314-BP).

Scientific Data Images for Recombinant Mouse Noggin Fc Chimera Protein, CF

Recombinant Mouse Noggin Fc Chimera Protein Bioactivity

Recombinant Mouse Noggin Fc Chimera Protein Bioactivity

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.06-0.3 µg/mL in the presence of 30 ng/mL of Recombinant Human BMP-4 (314-BP).

Formulation, Preparation, and Storage

719-NG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Noggin

Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse noggin shares 99% and 83% aa sequence identity with human and Xenopus noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

References

  1. Smith, W.C. and R.M. Harland (1992) Cell 70:829.
  2. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  3. Brunet, L.J. et al. (1998) Science 280:1455.

Alternate Names

NOG, SYM1, SYNS1, SYNS1A

Entrez Gene IDs

9241 (Human); 18121 (Mouse)

Gene Symbol

NOG

UniProt

U79163 (Mouse)

Additional Noggin Products

Product Documents for Recombinant Mouse Noggin Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse Noggin Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse Noggin Fc Chimera Protein, CF

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