Recombinant Mouse Notch-3 Fc Chimera Protein, CF

    
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  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse Notch‑3 Fc Chimera at 5 µg/mL (100 µL/well) can bind Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) with an apparent KD <5 nM.
  • Source
    Spodoptera frugiperda, Sf 21 (stably transfected)-derived
    Mouse Notch-3
    (Ala40-Glu468)
    Accession # Q61982
    IEGRMD Human IgG1
    (Pro100-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ala40
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    71.5 kDa (monomer)
  • SDS-PAGE
    90-95 kDa, reducing conditions
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1308-NT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Notch-3

Mouse Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes (1). The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The Notch molecule is synthesized as a 2318 amino acid (aa) precursor that contains an 39 aa signal sequence, a 1603 aa extracellular region, a 20 aa transmembrane (TM) segment and a 655 aa cytoplasmic domain. The large Notch extracellular domain has 34 EGF-like repeats followed by three notch/Lin-12 repeats (LNR) (2). The 11th and 12th EGF-like repeats of Notch have been shown to be both necessary and sufficient for binding the ligands Serrate and Delta, in Drosophila (3). Notch-3 has the same biochemical mechanism of signal tranduction as Notch-1, where a series of cleavage events result in the release of the Notch intracellular domain (NICD). NICD translocates into the nucleus and initiates transcription of Notch-responsive genes (4). Thus Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription.

Notch-3 is predominantly expressed in the developing central nervous system of mice (2). Mutations in Notch-3 in humans cause an autosomal dominant condition called CADASIL (cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy). This disorder is characterized by recurrent ischemic strokes at an early age without any underlying vascular risk and progressive dementia. Nearly all mutations leading to this disorder are clustered in the first 5 EGF repeats of the Notch-3 gene (5). Mouse Notch-3 shows 90% aa identity to human Notch-3 and 96% to rat Notch-3 over the entire protein.

  • References:
    1. Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
    2. Lardelli, M. et al. (1994) Mech Dev. 46:123.
    3. Rebay, I. et al. (1991) Cell 67:687.
    4. Mizutani, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9026.
    5. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
  • Entrez Gene IDs:
    4854 (Human); 18131 (Mouse)
  • Alternate Names:
    CADASIL; CASILneurogenic locus notch homolog protein 3; Notch (Drosophila) homolog 3; notch 3Notch homolog 3 (Drosophila); Notch homolog 3; Notch3; Notch-3
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Recombinant Rat Jagged 1 Fc Chimera Protein, CF

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