Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

Catalog # Availability Size / Price Qty
9048-B7-100

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Recombinant Mouse PD-L1/B7-H1 His-tag Protein Binding Activity
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Citations (2)
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Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-L1/B7-H1 is immobilized at 25 ng/mL (100 µL/well), the concentration of Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) that produces 50% of the optimal binding response is approximately 8-40 ng/mL
Source
Mouse myeloma cell line, NS0-derived mouse PD-L1/B7-H1 protein
Phe19-His239, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Phe19
Predicted Molecular Mass
26 kDa
SDS-PAGE
49-62 kDa, reducing conditions

Product Datasheets

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9048-B7

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9048-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse PD-L1/B7-H1 His-tag Protein Binding Activity View Larger

When Recombinant Mouse PD-L1/B7-H1 is immobilize at 25 ng/mL, it binds Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) with an ED50 of 8-40 ng/mL.

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Background: PD-L1/B7-H1

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules (1). Mature mouse B7-H1 consists of a 221 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 30 aa cytoplasmic domain (2). Within the ECD, mouse B7-H1 shares 73% and 86% aa sequence identity with human and rat B7-H1, respectively. B7-H1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (2-5), keratinocytes (6, 7), enothelial and intestinal epithelial cells (6, 8), as well as a variety of carcinomas and melanoma (9, 10). B7-H1 binds to T cell B7-1/CD80 and PD-1 (5, 6, 10-13). It suppresses T cell activation and proliferation (3, 6, 12, 14) and induces the apoptosis of activated T cells (9). It plays a role in the development of immune tolerance by promoting T cell anergy (5, 12) and enhancing regulatory T cell development (14). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (3, 8) and inhibits the development of Th17 cells (14). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (4, 7, 10, 13).

References
  1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  2. Tamura, H. et al. (2001) Blood 97:1809.
  3. Chen, L. et al. (2007) J. Immunol. 178:6634.
  4. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  5. Tsushima, F. et al. (2007) Blood 110:180.
  6. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  7. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  8. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  9. Dong, H. et al. (2002) Nat. Med. 8:793.
  10. Azuma, T. et al. (2008) Blood 111:3635.
  11. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  12. Park, J.-J. et al. (2010) Blood 116:1291.
  13. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  14. Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Citations for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. T cells use distinct topographical and membrane receptor scanning strategies that individually coalesce during receptor recognition
    Authors: E Cai, C Beppler, J Eichorst, K Marchuk, SW Eastman, MF Krummel
    Proceedings of the National Academy of Sciences of the United States of America, 2022-08-01;119(32):e2203247119.
    Species: Transgenic Mouse
    Sample Types: Whole Cell
    Applications: Bioassay
  2. Blockade of Tumor-Expressed PD-1 promotes lung cancer growth
    Authors: S Du, N McCall, K Park, Q Guan, P Fontina, A Ertel, T Zhan, AP Dicker, B Lu
    Oncoimmunology, 2018-01-29;7(4):e1408747.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF

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Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 12/21/2018
Application: In vitro bioactivity in cell culture

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 08/26/2018
Application: Immunoassay Standard

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 04/12/2018
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 03/30/2018
Application: In vivo study

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 03/15/2018
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 03/11/2018
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Amit Kumar on 12/18/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 11/27/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 11/18/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 11/15/2017
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 09/08/2017
Application: SDS-PAGE Control

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 07/20/2017
Application: Cell Adhesion

Recombinant Mouse PD-L1/B7-H1 His-tag Protein, CF
By Anonymous on 07/14/2017
Application: SPR chip ligand immobilization