Recombinant Mouse PD-1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1021-PD-100
Recombinant Mouse PD-1 Fc Chimera Protein Bioactivity
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Product Details
Citations (8)
FAQs
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Reviews (4)

Recombinant Mouse PD-1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-1 Fc Chimera at 1 µg/mL (100 µL/well) can bind Recombinant Mouse B7‑H1/PD‑L1 Fc Chimera (Catalog # 1019-B7) with an ED50 of 0.16-0.8 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse PD-1 protein
Mouse PD-1
(Leu25-Gln167)
Accession # Q02242
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Leu25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
43 kDa (monomer)
SDS-PAGE
66 kDa, reducing conditions

Product Datasheets

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1021-PD

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1021-PD

Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCL, NaCl and EDTA with Trehalose.
Reconstitution Reconstitute at 0.5 mg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse PD-1 Fc Chimera Protein Bioactivity View Larger

When Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) is coated at 1 µg/mL, Recombinant Mouse B7-H1/PD-L1 Fc Chimera (Catalog # 1019-B7) binds with an ED50 of 0.16-0.8 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: PD-1

Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors (1). Other members of this family include CD28, CTLA-4, ICOS, and BTLA (2-5). Mature mouse PD-1 consists of a 149 amino acid (aa) extracellular region (ECD) with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The mouse PD-1 ECD shares 65% aa sequence identity with the human PD-1 ECD. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important for mediating PD-1 signaling. PD-1 acts as a monomeric receptor and interacts in a 1:1 stoichiometric ratio with its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) (6, 7). PD‑1 is expressed on activated T cells, B cells, monocytes, and dendritic cells while PD-L1 expression is constitutive on the same cells and also on nonhematopoietic cells such as lung endothelial cells and hepatocytes (8, 9). Ligation of PD-L1 with PD-1 induces
co-inhibitory signals on T cells promoting their apoptosis, anergy, and functional exhaustion (10). Thus, the PD-1:PD-L1 interaction is a key regulator of the threshold of immune response and peripheral immune tolerance (11). Finally, blockade of the PD-1: PD-L1 interaction by either antibodies or genetic manipulation accelerates tumor eradication and shows potential for improving cancer immunotherapy (12, 13).

References
  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  5. Watanabe, N et al. (2003) Nat. Immunol. 4:670.
  6. Zhang, X. et al. (2004) Immunity 20:337.
  7. Lázár-Molnár, E. et al. (2008) Proc. Natl. Acad. Sci. USA 105:10483.
  8. Nishimura, H et al. (1996) Int. Immunol. 8:773.
  9. Keir, M.E. et al. (2008) Annu. Rev. Immunol. 26:677.
  10. Butte, M.J. et al. (2007) Immunity 27:111.
  11. Okazaki, T. et al. (2013) Nat. Immunol. 14:1212.
  12. Iwai, Y. et al. (2002) Proc. Natl. Acad. Sci. USA 99: 12293.
  13. Nogrady, B. (2014) Nature 513:S10.
Long Name
Programmed Death-1
Entrez Gene IDs
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

Citations for Recombinant Mouse PD-1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Co-Targeting Luminal B Breast Cancer with S-Adenosylmethionine and Immune Checkpoint Inhibitor Reduces Primary Tumor Growth and Progression, and Metastasis to Lungs and Bone
    Authors: A Mehdi, M Attias, A Arakelian, CA Piccirillo, M Szyf, SA Rabbani
    Cancers, 2022-12-22;15(1):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. The multi-specific VH-based Humabody CB213 co-targets PD1 and LAG3 on T cells to promote anti-tumour activity
    Authors: CJ Edwards, A Sette, C Cox, B Di Fiore, C Wyre, D Sydoruk, D Yadin, P Hayes, S Stelter, PD Bartlett, M Zuazo, MJ Garcia-Gra, G Benedetti, S Fiaska, NR Birkett, Y Teng, C Enever, H Arasanz, A Bocanegra, L Chocarro, G Fernandez, R Vera, B Archer, I Osuch, M Lewandowsk, YM Surani, G Kochan, D Escors, J Legg, AJ Pierce
    British Journal of Cancer, 2021-12-30;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay
  3. Dual and Opposite Costimulatory Targeting with a Novel Human Fusion Recombinant Protein Effectively Prevents Renal Warm Ischemia Reperfusion Injury and Allograft Rejection in Murine Models
    Authors: J Guiteras, L De Ramon, E Crespo, N Bolaños, S Barcelo-Ba, L Martinez-V, P Fontova, M Jarque, A Torija, O Bestard, D Resina, JM Grinyó, J Torras
    International Journal of Molecular Sciences, 2021-01-26;22(3):.
    Species: Rat
    Sample Types: In Vivo
    Applications: Surface Plasmon Resonance
  4. Long-Term Systemic Expression of a Novel PD-1 Blocking Nanobody from an AAV Vector Provides Antitumor Activity without Toxicity
    Authors: N Silva-Pili, E Martisova, MC Ballestero, S Hervas-Stu, N Casares, G González-S, C Smerdou, L Vanrell
    Biomedicines, 2020-12-02;8(12):.
    Species: Human
    Sample Types: Cell Culture Supernates, Recombinant Protein
    Applications: Bioassay, ELISA Capture
  5. DNA-Based Delivery of Checkpoint Inhibitors in Muscle and Tumor Enables Long-Term Responses with Distinct Exposure
    Authors: L Jacobs, E De Smidt, N Geukens, P Declerck, K Hollevoet
    Mol. Ther., 2020-02-13;0(0):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Standard
  6. Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity.
    Authors: Matsumura, Takuhiro, Sugawara, Yo, Yutani, Masahiro, Amatsu, Sho, Yagita, Hideo, Kohda, Tomoko, Fukuoka, Shin-Ich, Nakamura, Yutaka, Fukuda, Shinji, Hase, Koji, Ohno, Hiroshi, Fujinaga, Yukako
    Nat Commun, 2015-02-17;6(0):6255.
    Species: Mouse
    Sample Types: Protein
    Applications: Enzyme Assay
  7. Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses.
    Authors: Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ
    Immunity, 2007-07-12;27(1):111-22.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. BTNL2, a butyrophilin/B7-like molecule, is a negative costimulatory molecule modulated in intestinal inflammation.
    Authors: Arnett HA, Escobar SS, Gonzalez-Suarez E, Budelsky AL, Steffen LA, Boiani N, Zhang M, Siu G, Brewer AW, Viney JL
    J. Immunol., 2007-02-01;178(3):1523-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Recombinant Mouse PD-1 Fc Chimera Protein, CF

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Recombinant Mouse PD-1 Fc Chimera Protein, CF
By Anonymous on 12/02/2021
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-1 Fc Chimera Protein, CF
By Dong Hu on 11/08/2021
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-1 Fc Chimera Protein, CF
By Anonymous on 10/25/2021
Application: Binding assay/Protein-protein interaction

Recombinant Mouse PD-1 Fc Chimera Protein, CF
By Sarah Garrett on 02/11/2019
Application: Binding assay/Protein-protein interaction

0.5 ug mPD-1-hIgG1 (Cat# 1021-PD-100) was added to cells expressing mPD-L1 mCherry. Binding was detected with anti-human 488 secondary antibody.