>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-L2/B7-DC is coated at 0.4 µg/mL, 100 μL/well,
Mouse PD-1 Fc Chimera (Catalog # 1021-PD)
binds with a typical ED50 of 3-18 ng/mL.
Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein Leu20-Arg219, with a C-terminal 6-His tag
When Recombinant Mouse PD-L2/B7-DC (Catalog # 9107-PL) is coated at 0.4 µg/mL, Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) binds with a typical ED50 of 3-18 ng/mL.
Programmed Death Ligand 2 (PD-L2), also
known as B7-DC and butyrophilin-like protein, is a member of the B7 family of
proteins that provide signals for regulating T-cell activation and tolerance
(1). Mature mouse PD-L2 consists of a 199 amino acid (aa) extracellular domain
(ECD) with one V-like and one C-like Ig domain, a 23 aa transmembrane segment,
and a 5 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2
share 72% aa sequence identity. PD-L2 is expressed on dendritic cells, subsets
of activated CD4+ and CD8+ T cells, and memory B cells
that differentiate into plasma cells (3-5). At inflammatory sites such as
rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is
upregulated on synoviocytes, infiltrating macrophages, dendritic cells, and
airway epithelial cells (6-10). PD-L2, along with B7-H1/PD-L1, binds to T cell
PD-1 where it promotes IFN-gamma production and CD40 Ligand
up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition,
PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting
respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and IL-13
production, promotes IL-12 production by dendritic cells, and supports
allergen-induced airway hyper-responsiveness and mucus production (8, 10).
Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
Messal, N. et al. (2011) Mol. Immunol. 48:2214.
Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
Have you used Recombinant Mouse PD-L2/B7-DC His Tagged Protein, CF?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.