Recombinant Mouse PD-L2/B7-DC His Tagged Protein, CF Summary
Leu20-Arg219, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Mouse
PD-L2/B7-DC (Catalog #
9107-PL) is coated at 0.4 µg/mL, Recombinant Mouse PD-1 Fc Chimera (Catalog #
1021-PD) binds with a typical ED50 of 3-18 ng/mL.
Programmed Death Ligand 2 (PD-L2), also
known as B7-DC and butyrophilin-like protein, is a member of the B7 family of
proteins that provide signals for regulating T-cell activation and tolerance
(1). Mature mouse PD-L2 consists of a 199 amino acid (aa) extracellular domain
(ECD) with one V-like and one C-like Ig domain, a 23 aa transmembrane segment,
and a 5 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2
share 72% aa sequence identity. PD-L2 is expressed on dendritic cells, subsets
of activated CD4+ and CD8+ T cells, and memory B cells
that differentiate into plasma cells (3-5). At inflammatory sites such as
rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is
upregulated on synoviocytes, infiltrating macrophages, dendritic cells, and
airway epithelial cells (6-10).
PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and
IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).
- Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
- Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
- Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
- Messal, N. et al. (2011) Mol. Immunol. 48:2214.
- Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
- Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
- Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
- Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
- Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
- Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
- Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
- Shin, T. et al. (2003) J. Exp. Med. 198:31.
- Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
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