Recombinant Mouse PD-L2/B7-DC His Tagged Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse PD-L2/B7-DC is coated at 0.4 µg/mL, 100 μL/well, Recombinant Mouse PD-1 Fc Chimera (Catalog # 1021-PD) binds with a typical ED50 of 3-18 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived mouse PD-L2/B7-DC protein
    Leu20-Arg219, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu20
  • Predicted Molecular Mass
    23 kDa
  • SDS-PAGE
    28-52 kDa, reducing conditions
Product Datasheets

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9107-PL
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 200 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
When Recombinant Mouse
PD-L2/B7-DC (Catalog #
9107-PL) is coated at 0.4 µg/mL, Recombinant Mouse PD-1 Fc Chimera (Catalog #
1021-PD) binds with a typical ED50 of 3-18 ng/mL.
Background: PD-L2/B7-DC
Programmed Death Ligand 2 (PD-L2), also known as B7-DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1). Mature mouse PD-L2 consists of a 199 amino acid (aa) extracellular domain (ECD) with one V-like and one C-like Ig domain, a 23 aa transmembrane segment, and a 5 aa cytoplasmic domain (2, 3). Within the ECD, mouse and human PD-L2 share 72% aa sequence identity. PD-L2 is expressed on dendritic cells, subsets of activated CD4+ and CD8+ T cells, and memory B cells that differentiate into plasma cells (3-5). At inflammatory sites such as rheumatoid arthritis, allergen exposure, and virus infection, PD-L2 is upregulated on synoviocytes, infiltrating macrophages, dendritic cells, and airway epithelial cells (6-10).
PD-L2, along with B7-H1/PD-L1, binds to T cell PD-1 where it promotes IFN-gamma production and CD40 Ligand up-regulation while inhibiting IL-4 production (2, 3, 11, 12). In addition, PD-L2 binds to RGM-B on macrophages and alveolar epithelial cells, supporting respiratory immune tolerance (13). In asthma, PD-L2 suppresses IL-5 and
IL-13 production, promotes IL-12 production by dendritic cells, and supports allergen-induced airway hyper-responsiveness and mucus production (8, 10).
  • References:
    1. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
    2. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
    3. Tseng, S.-Y. et al. (2001) J. Exp. Med. 193:839.
    4. Messal, N. et al. (2011) Mol. Immunol. 48:2214.
    5. Zuccarino-Catania, G.V. et al. (2014) Nat. Immunol. 15:631.
    6. Guo, G. et al. (2012) Clin. Rheumatol. 31:271.
    7. Loke, P. and J.P. Allison (2003) Proc. Natl. Acad. Sci. USA 100:5336.
    8. Matsumoto, K. et al. (2004) J. Immunol. 172:2530.
    9. Stanciu, L.A. et al. (2006) J. Infec. Dis. 193:404.
    10. Lewkowich, I.P. et al. (2013) Mucosal Immun. 6:728.
    11. Ghiotto, M. et al. (2010) Int. Immunol. 22:651.
    12. Shin, T. et al. (2003) J. Exp. Med. 198:31.
    13. Xiao, Y. et al. (2014) J. Exp. Med. 211:943.
  • Long Name:
    Programmed Death-1 Ligand-2
  • Entrez Gene IDs:
    80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)
  • Alternate Names:
    B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PD-L2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2
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Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 28  
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His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
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His Tag APC-conjugated Antibody

ICFlow IC050A  
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His Tag Biotinylated Antibody

WB BAM050 4  
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His Tag PE-conjugated Antibody

ICFlow IC050P 3  
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His Tag Antibody

WB , Flow , AP MAB050R 2  
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His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
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His Tag PerCP-conjugated Antibody

ICFlow IC050C  
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His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
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Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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