Recombinant Mouse PDGF R alpha Fc Chimera Protein, CF

  (1 citations)     
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  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit the biological activity of PDGF-AB or PDGF-AA using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.01-0.04 µg/mL in the presence of 10 ng/mL recombinant human PDGF-AA.
  • Source
    Mouse myeloma cell line, NS0-derived mouse PDGF R alpha protein
    Mouse PDGF R alpha
    Leu25 - Glu524
    (Asp65Glu, Gly439Ala, Thr440Ala)
    Accession # P26618.3
    IEGRMD Human IgG1
    (Pro100 - Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu25
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    82.5 kDa (monomer)
  • SDS-PAGE
    125-140 kDa, reducing conditions
Product Datasheets

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1062-PR
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Background: PDGF R alpha

PDGF R alpha (platelet-derived growth factor receptor alpha) is a type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTK) (1 ‑ 3). PDGF R alpha and PDGF R beta can form homo- or hetero-dimeric receptors when engaged by dimers of the PDGF family of growth factors, which include disulfide-linked homodimers of PDGF-A, B, C or D, or the heterodimer PDGF-AB that is mainly found in human platelets. While multiple in vitro ligand-receptor combinations have been identified, in vivo evidence indicates that PDGF R alpha primarily binds PDGF-AA and PDGF-CC, while PDGF R beta primarily binds PDGF-BB and probably PDGF-DD. Like all class III RTKs, the extracellular domain (ECD) of mouse PDGF R alpha (aa 25 - 525) contains five immunoglobulin-like domains, while the intracellular region contains a split tyrosine kinase domain (aa 593 ‑ 954). Within the ECD, mouse PDGF R alpha shares 85%, 93%, 84%, 84%, and 81% aa sequence identity with human, rat, equine, canine and bovine PDGF R alpha respectively. PDGF R alpha autophosphorylates upon dimerization, activating signaling cascades in PI 3-kinase Ras-MAP kinase, and PLC-gamma pathways (1, 2). Signaling is down‑regulated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. PDGF R alpha is expressed at low levels in most mesenchymal cells, but is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture (1-3). During development, mesenchymal cells expressing PDGF R alpha respond to local gradients of epithelially produced PDGF-AA or PDGF-CC during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme (1, 4). Deletion of PDGF R alpha in mice severely impairs mesenchymal derivatives in both embryo and extraembryonic tissues, and high or low PDGF R alpha signaling in humans may result in spina bifida or cleft palate‑type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart and skin (scleroderma) (5 ‑ 7).

  • References:
    1. Andrae, J. et al. (2008) Genes Dev. 22:1276.
    2. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
    3. Do, M.S. et al. (1992) Oncogene 7:1567.
    4. Klinghoffer, R.A. et al. (2002) Dev. Cell 2:103.
    5. Martinho, O. (2009) Br. J. Cancer 101:973.
    6. Olson, L.E. and P. Soriano (2009) Dev. Cell 16:303.
    7. Baroni, S.S. et al. (2006) N. Engl. J. Med. 354:2667.
    • Long Name:
      Platelet-derived Growth Factor Receptor alpha
    • Entrez Gene IDs:
      5156 (Human); 18595 (Mouse)
    • Alternate Names:
      alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    1 Citations: Showing 1 - 1

    1. PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
      Authors: Yang X, Chrisman H, Weijer CJ
      Development, 2008;135(21):3521-30.
      Species: Human
      Sample Type: Recombinant Protein
      Application: Neut

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    Primary Antibodies
    Description Application Cat# Citations Images  

    Human IgG1 Antibody

    WB , ELISA MAB9894  
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    Human IgG1 Antibody

    WB MAB98941  
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    Recombinant Proteins
    Description Application Cat# Citations Images  

    Recombinant Human IgG1 Fc Protein, CF

    BA 110-HG 71
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    Reconstitution Buffers
    Description Application Cat# Citations Images  

    Reconstitution Buffer 1 (PBS)

    RB01
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