Recombinant Mouse PDGF R alpha Fc Chimera Protein, CF
Recombinant Mouse PDGF R alpha Fc Chimera Protein, CF Summary
|Mouse PDGF R alpha
Leu25 - Glu524
(Asp65Glu, Gly439Ala, Thr440Ala)
Accession # P26618.3
|IEGRMD||Human IgG1 |
(Pro100 - Lys330)
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: PDGF R alpha
PDGF R alpha (platelet-derived growth factor receptor alpha) is a type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTK) (1 ‑ 3). PDGF R alpha and PDGF R beta can form homo- or hetero-dimeric receptors when engaged by dimers of the PDGF family of growth factors, which include disulfide-linked homodimers of PDGF-A, B, C or D, or the heterodimer PDGF-AB that is mainly found in human platelets. While multiple in vitro ligand-receptor combinations have been identified, in vivo evidence indicates that PDGF R alpha primarily binds PDGF-AA and PDGF-CC, while PDGF R beta primarily binds PDGF-BB and probably PDGF-DD. Like all class III RTKs, the extracellular domain (ECD) of mouse PDGF R alpha (aa 25 - 525) contains five immunoglobulin-like domains, while the intracellular region contains a split tyrosine kinase domain (aa 593 ‑ 954). Within the ECD, mouse PDGF R alpha shares 85%, 93%, 84%, 84%, and 81% aa sequence identity with human, rat, equine, canine and bovine PDGF R alpha respectively. PDGF R alpha autophosphorylates upon dimerization, activating signaling cascades in PI 3-kinase Ras-MAP kinase, and PLC-gamma pathways (1, 2). Signaling is down‑regulated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. PDGF R alpha is expressed at low levels in most mesenchymal cells, but is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture (1-3). During development, mesenchymal cells expressing PDGF R alpha respond to local gradients of epithelially produced PDGF-AA or PDGF-CC during formation of the cranial and cardiac neural crest, retina, gonads, lung alveoli, intestinal villi, skin, hair follicles, skeleton, teeth, palate, and interstitial kidney mesenchyme (1, 4). Deletion of PDGF R alpha in mice severely impairs mesenchymal derivatives in both embryo and extraembryonic tissues, and high or low PDGF R alpha signaling in humans may result in spina bifida or cleft palate‑type malformations. Postnatally, PDGF R alpha is implicated in gliomas and fibrotic disorders of lung, heart and skin (scleroderma) (5 ‑ 7).
- Andrae, J. et al. (2008) Genes Dev. 22:1276.
- Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
- Do, M.S. et al. (1992) Oncogene 7:1567.
- Klinghoffer, R.A. et al. (2002) Dev. Cell 2:103.
- Martinho, O. (2009) Br. J. Cancer 101:973.
- Olson, L.E. and P. Soriano (2009) Dev. Cell 16:303.
- Baroni, S.S. et al. (2006) N. Engl. J. Med. 354:2667.
Citation for Recombinant Mouse PDGF R alpha Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
Authors: Yang X, Chrisman H, Weijer CJ
Sample Types: Recombinant Protein
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Mouse PDGF R alpha Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse PDGF R alpha Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Mouse PDGF R alpha Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image