Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus Protein, CF

(24 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.6-3 µg/mL.
  • Source
    E. coli-derived Cys25-Gly198, with a C-terminal 6-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    Cys25
  • Predicted Molecular Mass
    20 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
461-SH/CF
 
461-SH
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, Trehalose and with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus (Catalog # 461-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.6‑3 μg/mL.
1 μg/lane of Recombinant Mouse Sonic Hedgehog/Shh, N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
Background: Sonic Hedgehog/Shh
Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Mouse Shh encodes a 437 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  • References:
    1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
    2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
    3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
    4. Echelard, Y. et al. (1993) Cell 75:1417.
    5. Zeng, X. et al. (2001) Nature 411:716.
    6. Feng, J. et al. (2004) Development 131:4357.
    7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
    8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
    9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
    10. Etheridge, L.A. et al. (2010) Development 137:133.
    11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
    12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
    13. Lewis, P.M. et al. (2001) Cell 105:599.
    14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
    15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
    16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
  • Entrez Gene IDs:
    6469 (Human); 20423 (Mouse)
  • Alternate Names:
    HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. TGFbeta signaling in myeloid cells regulates mammary carcinoma cell invasion through fibroblast interactions.
    Authors: Shaw A, Pickup M, Chytil A, Aakre M, Owens P, Moses H, Novitskiy S
    PLoS ONE, 2015;10(1):e0117908.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Deficiency of NOX1 or NOX4 Prevents Liver Inflammation and Fibrosis in Mice through Inhibition of Hepatic Stellate Cell Activation.
    Authors: Lan T, Kisseleva T, Brenner D
    PLoS ONE, 2015;10(7):e0129743.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Modulation of Ciliary Phosphoinositide Content Regulates Trafficking and Sonic Hedgehog Signaling Output.
    Authors: Chavez M, Ena S, Van Sande J, de Kerchove d'Exaerde A, Schurmans S, Schiffmann S
    Dev Cell, 2015;34(3):338-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Deletion of IFT80 Impairs Epiphyseal and Articular Cartilage Formation Due to Disruption of Chondrocyte Differentiation.
    Authors: Yuan X, Yang S
    PLoS ONE, 2015;10(6):e0130618.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Generation of rat-induced pluripotent stem cells from a new model of metabolic syndrome.
    Authors: Takenaka-Ninagawa, Nana, Kawabata, Yuka, Watanabe, Shogo, Nagata, Kohzo, Torihashi, Shigeko
    PLoS ONE, 2014;9(8):e104462.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  6. The fate of the primary cilium during myofibroblast transition.
    Authors: Rozycki M, Lodyga M, Lam J, Miranda M, Fatyol K, Speight P, Kapus A
    Mol Biol Cell, 2014;25(5):643-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. MicroRNA-17-92 cluster mediates the proliferation and survival of neural progenitor cells after stroke.
    Authors: Liu, Xian Shu, Chopp, Michael, Wang, Xin Li, Zhang, Li, Hozeska-Solgot, Ann, Tang, Tao, Kassis, Haifa, Zhang, Rui Lan, Chen, Charles, Xu, Jennifer, Zhang, Zheng Ga
    J Biol Chem, 2013;288(18):12478-88.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: In Vivo
  8. The acid-secreting parietal cell as an endocrine source of Sonic Hedgehog during gastric repair.
    Authors: Engevik A, Feng R, Yang L, Zavros Y
    Endocrinology, 2013;154(12):4627-39.
    Species: Mouse
    Sample Type: In Vivo
  9. A modular gain-of-function approach to generate cortical interneuron subtypes from ES cells.
    Authors: Au E, Ahmed T, Karayannis T, Biswas S, Gan L, Fishell G
    Neuron, 2013;80(5):1145-58.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord.
    Authors: Belgacem YH, Borodinsky LN,
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(11):4482-7.
    Species: Xenopus
    Sample Type: Whole Tissue
    Application: Bioassay
  11. Notch and Wnt signaling mediated rod photoreceptor regeneration by Muller cells in adult mammalian retina.
    Authors: Del Debbio CB, Balasubramanian S, Parameswaran S, Chaudhuri A, Qiu F, Ahmad I
    PLoS ONE, 2010;5(8):e12425.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  12. Establishment and characterization of immortalized Gli-null mouse embryonic fibroblast cell lines.
    Authors: Lipinski RJ, Bijlsma MF, Gipp JJ, Podhaizer DJ, Bushman W
    BMC Cell Biol., 2008;9(0):49.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(8):2997-3002.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Sonic hedgehog promotes stem-cell potential of Muller glia in the mammalian retina.
    Authors: Wan J, Zheng H, Xiao HL, She ZJ, Zhou GM
    Biochem. Biophys. Res. Commun., 2007;363(2):347-54.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  15. Regulation of cavernous nerve injury-induced apoptosis by sonic hedgehog.
    Authors: Podlasek CA, Meroz CL, Tang Y, McKenna KE, McVary KT
    Biol. Reprod., 2007;76(1):19-28.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  16. Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death.
    Authors: Yamashita H, Nakamura T, Takahashi T, Nagano Y, Hiji M, Hirabayashi T, Amano T, Yagi T, Sakai N, Kohriyama T, Matsumoto M
    J. Neurochem., 2006;98(1):45-56.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Expression of the short stature homeobox gene Shox is restricted by proximal and distal signals in chick limb buds and affects the length of skeletal elements.
    Authors: Tiecke E, Bangs F, Blaschke R, Farrell ER, Rappold G, Tickle C
    Dev. Biol., 2006;298(2):585-96.
  18. An early role for sonic hedgehog from foregut endoderm in jaw development: ensuring neural crest cell survival.
    Authors: Brito JM, Teillet MA, Le Douarin NM
    Proc. Natl. Acad. Sci. U.S.A., 2006;103(31):11607-12.
    Species: Avian - Quail
    Sample Type: Whole Tissue
    Application: Bioassay
  19. Multipotentiality, homing properties, and pyramidal neurogenesis of CNS-derived LeX(ssea-1)+/CXCR4+ stem cells.
    Authors: Corti S, Locatelli F, Papadimitriou D, Donadoni C, Del Bo R, Fortunato F, Strazzer S, Salani S, Bresolin N, Comi GP
    FASEB J., 2005;19(13):1860-2.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. FGF8 dose-dependent regulation of embryonic submandibular salivary gland morphogenesis.
    Authors: Jaskoll T, Witcher D, Toreno L, Bringas P, Moon AM, Melnick M
    Dev. Biol., 2004;268(2):457-69.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. Derivation of midbrain dopamine neurons from human embryonic stem cells.
    Authors: Perrier AL, Tabar V, Barberi T, Rubio ME, Bruses J, Topf N, Harrison NL, Studer L
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(34):12543-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  22. Shh and Fgf8 act synergistically to drive cartilage outgrowth during cranial development.
    Authors: Abzhanov A, Tabin CJ
    Dev. Biol., 2004;273(1):134-48.
    Species: Chicken
    Sample Type: Whole Tissue
    Application: Bioassay
  23. Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2.
    Authors: Viti J, Gulacsi A, Lillien L
    J. Neurosci., 2003;23(13):5919-27.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  24. Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium.
    Authors: Mistretta CM, Liu HX, Gaffield W, MacCallum DK
    Dev. Biol., 2003;254(1):1-18.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 24 Citations
Primary Antibodies
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His Tag Alexa Fluor® 488-conjugated Antibody

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Protein Purification Kits
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Histidine-tagged Protein Purification Resin

IP999 1
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