Recombinant Mouse ST6GALNAC2 Protein, CF

Catalog # Availability Size / Price Qty
R&D Systems Recombinant Proteins and Enzymes
1 Image
Product Details
Citations (1)
Supplemental Products

Recombinant Mouse ST6GALNAC2 Protein, CF Summary

Learn more about Fluorescent Glycan Labeling and Detection

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum. The specific activity is >700 pmol/min/μg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived mouse ST6 Sialyltransferase 2/ST6GALNAC2 protein
Ser28-Arg373, with a C-terminal 6‑His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
40 kDa
40-50 kDa, reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

  • Sialyltransferase Activity Kit (Catalog # EA002)
  • 10X Assay Buffer (supplied in kit): 250 mM Tris, pH 7.5
  • Recombinant Mouse Sialyltransferase 2/ST6GALNAC2  (rmST6GALNAC2) (Catalog # 6468-GT)
  • CMP-Neu5Ac (Sigma, Catalog # C8271), 10 mM stock in deionized water
  • Fetuin (Sigma, Catalog # F3385), 50 mg/mL stock in deionized water
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent

  1. Prepare 1X Assay Buffer containing 10 mM MnCl2 by combining equal volumes of 10X Assay Buffer and 100 mM MnCl2 and diluting 5-fold with deionized water.
  2. Dilute the 1 mM Phosphate Standard (provided in kit) by adding 40 μL of the 1 mM Standard to 360 μL of 1X Assay Buffer for a 100 μM stock.  This is the first point of the standard curve.
  3. Complete the standard curve by performing six one-half serial dilutions of the 100 μM Phosphate stock using 1X Assay Buffer. The standard curve has a range of 0.078 to 5 nmol per well.
  4. Prepare a reaction mixture containing 0.5 mM CMP-Neu5Ac, 20 mg/mL Fetuin, and 4 μg/mL Coupling Phosphatase 2 in 1X Assay Buffer.
  5. Dilute rmST6GALNAC2 to 2.4 µg/mL in 1X Assay Buffer.
  6. Load 50 µL of each dilution of the standard curve into a plate. Include a curve blank containing 50 μL of 1X Assay Buffer.
  7. Load 25 µL of the 2.4 µg/mL rmST6GALNAC2 into empty wells of the same plate containing the standard curve. Include a Control containing 25 µL of 1X Assay Buffer.
  8. Add 25 µL of reaction mixture to the wells, excluding the standard curve.
  9. Cover the plate with parafilm or a plate sealer and incubate at 37 °C for 20 minutes.
  10. Add 30 µL of the Malachite Green Reagent A to all wells. Mix and incubate for 10 minutes at room temperature.
  11. Add 100 µL of deionized water to all wells.
  12. Add 30 µL of the Malachite Green Reagent B to all wells. Mix and incubate for 20 minutes at room temperature.
  13. Read plate at 620 nm (absorbance) in endpoint mode.
  14. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Phosphate released* (nmol) x (1000 pmol/nmol)
Incubation time (min) x amount of enzyme (µg)

     *Derived from the phosphate standard curve using linear fitting and adjusted for Control.

Per Reaction:
  • rmST6GALNAC2: 0.060 μg
  • Coupling Phosphatase 2: 0.1 μg
  • CMP-Neu5Ac: 250 µM
  • Fetuin: 0.5 mg
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: ST6 Sialyltransferase 2/ST6GALNAC2

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion. Sialyltransferases are key enzymes that regulate the serum levels of sialic acid‑containing molecules. Alpha‑N‑acetylgalactosaminide alpha ‑2,6‑sialyltransferase 2, encoded by the ST6GALNAC2 gene, is a type II membrane protein localized in the Golgi network and catalyzes 2,6‑sialylation of the Tn antigen (GalNAc‑O‑Ser/Thr) (1). Sialyl‑Tn antigen is highly expressed in several human carcinomas and is associated with carcinoma aggressiveness and poor prognosis. Cells transfected with ST6GALNAC2 synthesized the sialyl‑6T structure [Gal beta 1‑3 (Neu5Ac alpha 2‑6)GalNAc‑O‑Ser/Thr] (2). In vitro analysis revealed that the enzyme can use both Gal beta 1‑3GalNAc and Neu5Ac alpha 2‑3Gal beta 1-3GalNAc  as acceptor substrates (3). Recently, it has been reported that disregulation of this gene is involved in the pathogenesis of IgA nephropathy, a common kidney disease that occurs when antibody IgA accumulates in kidneys (4). The enzyme may also be used to synthesize a cancer vaccine (5, 6). At the protein level, mouse ST6GALNAC2 shows 75% amino acid sequence identity with the human homologue. The activity of this enzyme has been measured using a phosphatase‑coupled assay (7).

  1. Kono, M., et al. (2000) Biochem. Biophys. Res. Commun. 272:94.
  2. Marcos, N.T., et al. (2004) Cancer Res. 64:7050.
  3. Samyn-Petit, B., et al. (2000) Biochim. Biophys. Acta 1474:201.
  4. Li, G.S., et al. (2007) Hum. Mutat. 28:950.
  5. Julien, S., et al. (2009) Br. J. Cancer 100:1746.
  6. Sewell, R., et al. (2006) J. Biol. Chem. 281:3586.
  7. Wu, Z.L., et al. (2011) Glycobiology 21:727.
Long Name
ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 2
Entrez Gene IDs
10610 (Human)
Alternate Names
(alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B; alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2; EC 2.4.99.-; FLJ45660; GalNAc alpha-2,6-sialyltransferase II; SAITL1; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) B; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase); Sialyltransferase 7B; sialyltransferase-like 1; SIAT7; SIAT7B; SIAT7-B; SIATL1; ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 2; ST6GalNAc II; ST6GALNAC2; ST6GalNAcII; ST6GalNAII; STHM; STHM(alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase

Citation for Recombinant Mouse ST6GALNAC2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Structural basis of mammalian mucin processing by the human gut O-glycopeptidase OgpA from Akkermansia muciniphila
    Authors: B Trastoy, A Naegeli, I Anso, J Sjögren, ME Guerin
    Nat Commun, 2020;11(1):4844.
    Species: E. Coli
    Sample Types: Peptide
    Applications: Bioassay


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Glycan Detection Reagents

CMP-Cy3-Sialic Acid

CMP-Cy5-Sialic Acid

Glycosyltransferase Activity Assays

Other ELISA and Assay Kits

Recombinant Enzymes

Reviews for Recombinant Mouse ST6GALNAC2 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse ST6GALNAC2 Protein, CF and earn rewards!

Have you used Recombinant Mouse ST6GALNAC2 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review