Recombinant Mouse ST6GALNAC2 Protein, CF

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6468-GT-020
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Recombinant Mouse ST6GALNAC2 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to transfer Neu5Ac from CMP-Neu5Ac to fetuin of fetal calf serum. The specific activity is >700 pmol/min/μg, as measured under the described conditions.
Source
Mouse myeloma cell line, NS0-derived mouse ST6 Sialyltransferase 2/ST6GALNAC2 protein
Ser28-Arg373, with a C-terminal 6‑His tag
Accession #
N-terminal Sequence
Analysis
Ser28
Predicted Molecular Mass
40 kDa
SDS-PAGE
40-50 kDa, reducing conditions

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Background: ST6 Sialyltransferase 2/ST6GALNAC2

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion. Sialyltransferases are key enzymes that regulate the serum levels of sialic acid‑containing molecules. Alpha‑N‑acetylgalactosaminide alpha ‑2,6‑sialyltransferase 2, encoded by the ST6GALNAC2 gene, is a type II membrane protein localized in the Golgi network and catalyzes 2,6‑sialylation of the Tn antigen (GalNAc‑O‑Ser/Thr) (1). Sialyl‑Tn antigen is highly expressed in several human carcinomas and is associated with carcinoma aggressiveness and poor prognosis. Cells transfected with ST6GALNAC2 synthesized the sialyl‑6T structure [Gal beta 1‑3 (Neu5Ac alpha 2‑6)GalNAc‑O‑Ser/Thr] (2). In vitro analysis revealed that the enzyme can use both Gal beta 1‑3GalNAc and Neu5Ac alpha 2‑3Gal beta 1-3GalNAc  as acceptor substrates (3). Recently, it has been reported that disregulation of this gene is involved in the pathogenesis of IgA nephropathy, a common kidney disease that occurs when antibody IgA accumulates in kidneys (4). The enzyme may also be used to synthesize a cancer vaccine (5, 6). At the protein level, mouse ST6GALNAC2 shows 75% amino acid sequence identity with the human homologue. The activity of this enzyme has been measured using a phosphatase‑coupled assay (7).

References
  1. Kono, M., et al. (2000) Biochem. Biophys. Res. Commun. 272:94.
  2. Marcos, N.T., et al. (2004) Cancer Res. 64:7050.
  3. Samyn-Petit, B., et al. (2000) Biochim. Biophys. Acta 1474:201.
  4. Li, G.S., et al. (2007) Hum. Mutat. 28:950.
  5. Julien, S., et al. (2009) Br. J. Cancer 100:1746.
  6. Sewell, R., et al. (2006) J. Biol. Chem. 281:3586.
  7. Wu, Z.L., et al. (2011) Glycobiology 21:727.
Long Name
ST6 alpha-N-acetylGalactosaminide alpha-2,6-sialyltransferase 2
Entrez Gene IDs
10610 (Human)
Alternate Names
(alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B; alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 2; EC 2.4.99.-; FLJ45660; GalNAc alpha-2,6-sialyltransferase II; SAITL1; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminide B; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase) B; sialyltransferase 7((alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase); Sialyltransferase 7B; sialyltransferase-like 1; SIAT7; SIAT7B; SIAT7-B; SIATL1; ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminidealpha-2,6-sialyltransferase 2; ST6GalNAc II; ST6GALNAC2; ST6GalNAcII; ST6GalNAII; STHM; STHM(alpha-N-acetylneuraminyl-2,3-beta-galactosyl-1,3)-N-acetyl galactosaminidealpha-2,6-sialyltransferase

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