Sialyltransferase Activity Kit
Sialyltransferase Activity Kit Summary
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- Applicable for all sialyltransferases
- No time-consuming separation steps (e.g. column chromatography)
- Provides accurate kinetic analysis of sialyltransferase reactions
- Amenable to high-throughput analysis
- CHO cell-expressed Recombinant Human CD73
- Phosphatase Buffer 2
- 100 mM MnCl2
- Phosphate Standard
- Malachite Green Reagents A & B
Activity of ST6GALNAC2
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The ability of Recombinant Mouse ST6 Sialyltransferase 2/ST6GALNAC2 (Catalog # 6468-GT) to transfer Neu5Ac (sialic acid) from CMP-Neu5Ac to bovine asialofetuin was measured using the Sialyltransferase Activity Kit (Catalog # EA002). The activity was plotted against enzyme input, and the specific activity of ST6GALNAC2 was determined to be 774 pmol/min/μg.
Citations for Sialyltransferase Activity Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Engineering of CHO cells for the production of vertebrate recombinant sialyltransferases
Authors: B Houeix, MT Cairns
PeerJ, 2019;7(0):e5788. 2019
Transfer of Functional Cargo in Exomeres
Authors: Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey
Cell Rep, 2019;0(0):. 2019
B-cell-independent sialylation of IgG
Authors: Mark B Jones
Proc Natl Acad Sci USA, 2016;113(26):7207-12. 2016
Mutation in ST6GALNAC5 identified in family with coronary artery disease.
Authors: InanlooRahatloo, Kolsoum, Parsa, Amir Far, Huse, Klaus, Rasooli, Paniz, Davaran, Saeid, Platzer, Matthias, Kramer, Marcel, Fan, Jian-Bin, Turk, Casey, Amini, Sasan, Steemers, Frank, Gunderson, Kevin, Ronaghi, Mostafa, Elahi, Elahe
Sci Rep, 2014;4(0):3595. 2014
Selective exo-enzymatic labeling of N-glycans on the surface of living cells by recombinant ST6Gal I.
Authors: Mbua N, Li X, Flanagan-Steet H, Meng L, Aoki K, Moremen K, Wolfert M, Steet R, Boons G
Angew Chem Int Ed Engl, 2013;52(49):13012-5. 2013
Does the Malachite Green Phosphate Detection Kit (DY996) detect both pyrophosphate (diphosphate) and free phosphate (monophosphate)?
The Malachite Green Phosphate Detection Kit measures free inorganic phosphate in aqueous solutions. The assay principle is based on complex formation between malachite green molybdate and free phosphate under acidic conditions. Lipid bound, protein-bound, pyrophashate and other condensed phosphates must be hydrolyzed prior to measurement in the malachite green assay.
Phosphatase Activity Assays
Supplemental ELISA Products
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