Sialyltransferase Activity Kit

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The Sialyltransferase Activity Kit (Catalog # EA002) provides a simple, non-radioactive high-throughput compatible format for assaying the enzyme activity of all sialyltransferases regardless of different acceptor substrates. This kit utilizes the 5’-nucleotidase CD73 as a coupling phosphatase to remove inorganic phosphate quantitatively from the leaving nucleotide cytidine 5’-monophosphate (CMP) that is generated during sialyltransferase reactions.

Features

• Applicable for all sialyltransferases
• Non-radioactive
• No time-consuming separation steps (e.g. column chromatography)
• Provides accurate kinetic analysis of sialyltransferase reactions
• Amenable to high-throughput analysis

Kit Contents

• CHO cell-expressed Recombinant Human CD73
• CMP
• Phosphatase Buffer 2
• 100 mM MnCl₂
• Phosphate Standard
• Malachite Green Reagents A & B

Data Example

Activity of ST6GALNAC2

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The ability of Recombinant Mouse ST6 Sialyltransferase 2/ST6GALNAC2 (Catalog # 6468-GT) to transfer Neu5Ac (sialic acid) from CMP-Neu5Ac to bovine asialofetuin was measured using the Sialyltransferase Activity Kit (Catalog # EA002). The activity was plotted against enzyme input, and the specific activity of ST6GALNAC2 was determined to be 774 pmol/min/μg.