Recombinant Mouse sTNF RI/TNFRSF1A Protein

Carrier Free

Catalog # Availability Size / Price Qty
425-R1-050/CF

With Carrier

Catalog # Availability Size / Price Qty
425-R1-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (15)
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Recombinant Mouse sTNF RI/TNFRSF1A Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 0.1-0.6 µg/mL in the presence of 0.1 ng/mL of recombinant mouse TNF-alpha.
Source
E. coli-derived mouse TNF RI/TNFRSF1A protein
Ile22-Ala212, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
21 kDa

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425-R1 (with carrier)

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425-R1/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

425-R1

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

425-R1/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R-p55/p60 and TNFRSF1A) is a 55 kDa type I transmembrane protein member of the TNF receptor superfamily, designated TNFRSF1A (1, 2). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence, a 191 aa extracellular domain (ECD) with a PLAD (pre-ligand assembly domain) that mediates constitutive dimer/trimer formation, followed by four CRD (cysteine-rich domains), a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (3, 4). The ECD of mouse TNF RI shares 70%, 88%, 67%, 70% and 64% aa sequence identity with human, rat, canine, feline and porcine TNF RI, respectively. Both TNF RI and TNF RII (TNFRSF1B) are widely expressed and contain four TNF-alpha trimer-binding CRD in their ECD. However, TNF RI is thought to mediate most of the cellular effects of TNF-alpha (3). It is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria (1, 2, 5). TNF RI is also a receptor for TNF-beta /TNFSF1B (lymphotoxin-alpha ) (6). TNF RI is stored in the Golgi and translocates to the cell surface following pro-inflammatory stimuli (7). TNF-alpha stabilizes TNF RI and induces its sequestering in lipid rafts, where it activates NF kappa B and is cleaved by ADAM-17/TACE (8, 9, 16). Release of the 28-34 kDa TNF RI ECD also occurs constitutively and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 10-12). Full-length TNF RI may also be released in exosome-like vesicles (13). Release helps to resolve inflammatory reactions, since it down-regulates cell surface TNF RI and provides soluble TNF RI to bind TNF-alpha (10, 14, 15). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (1). Mutations of human TNF R1 can result in inflammatory episodes known as TRAPS (TNFR-associated periodic syndrome) (7).

References
  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Chan, F.K. et al. (2000) Science 288:2351.
  4. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
  5. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  6. Banner, D.W et al. (1993) Cell 73: 431.
  7. Turner, M.D. et al. (2012) Biosci. Rep. 32:105.
  8. Legler, D.F. et al. (2003) Immunity 18:655.
  9. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  10. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  11. Jin, L. et al. (2000) J. Immunol. 165:5153.
  12. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  13. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  14. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  15. McDermott, M.F. et al. (1999) Cell 97:133.
Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

Citations for Recombinant Mouse sTNF RI/TNFRSF1A Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. A wake-like state in vitro induced by transmembrane TNF/soluble TNF receptor reverse signaling
    Authors: C Dykstra-Ai, KMS Koh, J Nguyen, M Xue, S Roy, JM Krueger
    Brain, Behavior, and Immunity, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Scavenging Tumor Necrosis Factor alpha Does Not Affect Inhibition of Dentate Granule Cells Following In Vitro Entorhinal Cortex Lesion
    Authors: D Kleidonas, A Vlachos
    Cells, 2021;10(11):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  3. Macrophages employ quorum licensing to regulate collective activation
    Authors: JJ Muldoon, Y Chuang, N Bagheri, JN Leonard
    Nat Commun, 2020;11(1):878.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Pseudomonas aeruginosa quorum-sensing metabolite induces host immune cell death through cell surface lipid domain dissolution
    Authors: D Song, J Meng, J Cheng, Z Fan, P Chen, H Ruan, Z Tu, N Kang, N Li, Y Xu, X Wang, F Shu, L Mu, T Li, W Ren, X Lin, J Zhu, X Fang, MW Amrein, W Wu, LT Yan, J Lü, T Xia, Y Shi
    Nat Microbiol, 2018;0(0):.
    Species: Mouse
    Sample Types:
    Applications: In Vivo
  5. Pharmacokinetic and Pharmacodynamic Characterisation of an Anti-Mouse TNF Receptor 1 Domain Antibody Formatted for In Vivo Half-Life Extension.
    Authors: Goodall L, Ovecka M, Rycroft D, Friel S, Sanderson A, Mistry P, Davies M, Stoop A
    PLoS ONE, 2015;10(9):e0137065.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  6. Generation and characterization of small single domain antibodies inhibiting human tumor necrosis factor receptor 1.
    Authors: Steeland S, Puimege L, Vandenbroucke R, Van Hauwermeiren F, Haustraete J, Devoogdt N, Hulpiau P, Leroux-Roels G, Laukens D, Meuleman P, De Vos M, Libert C
    J Biol Chem, 2015;290(7):4022-37.
    Species: Human
    Sample Types: Complex Sample Type
    Applications: Enzyme Assay
  7. Glial tumor necrosis factor alpha (TNF-alpha) generates metaplastic inhibition of spinal learning.
    Authors: Huie JR, Baumbauer KM, Lee KH, Bresnahan JC, Beattie MS, Ferguson AR, Grau JW
    PLoS ONE, 2012;7(6):e39751.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. Cold pre-conditioning neuroprotection depends on TNF-alpha and is enhanced by blockade of interleukin-11.
    Authors: Mitchell HM, White DM, Domowicz MS, Kraig RP
    J. Neurochem., 2011;117(2):187-96.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  9. Tumor necrosis factor-alpha mediates one component of competitive, experience-dependent plasticity in developing visual cortex.
    Authors: Kaneko M, Stellwagen D, Malenka RC, Stryker MP
    Neuron, 2008;58(5):673-80.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  10. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006;177(3):1967-74.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  11. P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins.
    Authors: Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P
    J. Biol. Chem., 2006;281(41):30684-96.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  13. Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
    Authors: LaBue M, Colburn KK, Green LM
    Thyroid, 2004;14(4):249-62.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  14. A role for interferon-gamma in focal cerebral ischemia in mice.
    Authors: Lambertsen KL, Gregersen R, Meldgaard M, Clausen BH, Heibol EK, Ladeby R, Knudsen J, Frandsen A, Owens T, Finsen B
    J. Neuropathol. Exp. Neurol., 2004;63(9):942-55.
  15. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2004;231(1):103-11.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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