Recombinant Mouse TLR7 Protein, CF

Catalog # Availability Size / Price Qty
9568-TR-050
The Innovator Series.
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Recombinant Mouse TLR7 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
E. coli-derived mouse TLR7 protein
Asn275-Phe444, with an N-terminal Met and a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Met and Met313
Predicted Molecular Mass
21 kDa
SDS-PAGE
16 kDa and 21 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9568-TR

Formulation Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution Reconstitute at 200 μg/mL in 10 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity not tested The Innovator Series. View Larger

R&D Systems proteins are almost always sold with a bioassay to indicate activity. However, we recognize that sometimes proteins might be novel, and their bioactivity may not be well understood. In addition, some researchers may wish to use polypeptides to make antibodies. To facilitate the advancement of new science, we now offer our Innovator Series of proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TLR7

TLR7 (Toll-like receptor 7) is a member of the Toll-like receptor family of innate immune receptors. It is mainly expressed in lung, placenta and spleen and belongs to a new sub-family of the TLRs comprising of TLR7, TLR8, an TLR9 (1, 2). Mouse TLR7 cDNA encodes a 1050 amino acid (aa) precursor that contains a 26 aa signal sequence, an 811 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 192 aa cytoplasmic domain. Mouse TLR7 is 81% aa identical to mature human TLR7. TLR7 is located in the endosomes and involved in the sequence-dependent sensing of single-stranded RNA, thereby detecting bacterial or viral nucleic acids (3, 4, 5, 6).

References
  1. Du, X. et al. (2000) Eur. Cytokine Netw. 11:362.
  2. Chuang, T.H. and R.J. Ulevitch (2000) Eur. Cytokine Netw. 11:372.
  3. Hornung, V. et al. (2002) J. Immunol. 168:4531.
  4. Melchjorsen, J. et al. (2005) J. Virol. 79:12944.
  5. Gantier M.P. et al. (2008) J. Immunol. 180:2117.
  6. Kim Y.M. et al. (2008) Nature 452:234.
Long Name
Toll-like Receptor 7
Entrez Gene IDs
51284 (Human); 170743 (Mouse); 317468 (Rat)
Alternate Names
TLR7; toll-like receptor 7

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