Recombinant Mouse TLR7 Protein, CF

Name: Recombinant Mouse TLR7 Protein, CF
Brand: R&D Systems
SKU: 9568-TR
Price: 716 USD
Availability: InStock

R&D Systems | Catalog # 9568-TR

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems E. coli-derived Recombinant Mouse TLR7 Protein (9568-TR)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P58681

View all TLR7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived mouse TLR7 protein
Asn275-Phe444, with an N-terminal Met and a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met and Met313

Predicted Molecular Mass

21 kDa

SDS-PAGE

16 kDa and 21 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation, and Storage

9568-TR

Formulation	Lyophilized from a 0.2 μm filtered solution in HCl.
Reconstitution	Reconstitute at 200 μg/mL in 10 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TLR7

TLR7 (Toll-like receptor 7) is a member of the Toll-like receptor family of innate immune receptors. It is mainly expressed in lung, placenta and spleen and belongs to a new sub-family of the TLRs comprising of TLR7, TLR8, an TLR9 (1, 2). Mouse TLR7 cDNA encodes a 1050 amino acid (aa) precursor that contains a 26 aa signal sequence, an 811 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 192 aa cytoplasmic domain. Mouse TLR7 is 81% aa identical to mature human TLR7. TLR7 is located in the endosomes and involved in the sequence-dependent sensing of single-stranded RNA, thereby detecting bacterial or viral nucleic acids (3, 4, 5, 6).