Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

R&D Systems | Catalog # 721-DR

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein (721-DR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

Q6GSD9

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TRAIL R2/TNFRSF10B Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse TRAIL R2/TNFRSF10B protein
Mouse TRAIL R2
(Asn53 - Ser177) &
(Arg58 - Ser177)
Accession # Q6GSD9		 IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn53 & Arg58

Predicted Molecular Mass

40 kDa (monomer)

SDS-PAGE

49 kDa, reducing conditions

Activity

Measured by its ability to inhibit TRAIL-mediated cytotoxicity using L‑929 mouse fibroblast cells treated with TRAIL.
The ED50 for this effect is 3‑12 ng/mL in the presence of 20 ng/mL of rhTRAIL or 12 ng/mL cross-linked rhTRAIL (Catalog # 375-TL and MAB050).

Formulation, Preparation, and Storage

721-DR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL R2/TNFRSF10B

Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). Mouse TRAIL R2 cDNA encodes a 381 amino acid residue precursor protein containing an extracellular cysteine-rich domain, a transmembrane domain and a cytoplasmic death domain. Human and mouse TRAIL R2 share 49% amino acid sequence similarity. The death domains of human TRAIL R1 and TRAIL R2 share high homology with the death domain of mouse TRAIL R2, 76% and 79%, respectively. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The mouse TRAIL R2/Fc chimera neutralizes the ability of rhTRAIL to induce apoptosis. Besides the death domain containing receptors TRAIL R2 and TRAIL R1/DR4, three TRAIL decoy receptors, TRAIL R3/DcR1, TRAIL R4/DcR2, and OPG, have been reported.

References

  1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
  2. Walczak, H. et al. (1997) EMBO J. 16:5386.
  3. Golstein, P. (1997) Curr. Biol. 7:R750.
  4. Wu, G.S. et al. (1999) Cancer Research 59:2770.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Entrez Gene IDs

8795 (Human); 21933 (Mouse)

Gene Symbol

TNFRSF10B

UniProt

Q6GSD9

Additional TRAIL R2/TNFRSF10B Products

Product Documents for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

For research use only

Citations for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

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FAQs for Recombinant Mouse TRAIL R2/TNFRSF10B Fc Chimera Protein, CF

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  • Q:

    This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    A: The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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