Recombinant Mouse Trypsin 3/PRSS3 Protein, CF

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Recombinant Mouse Trypsin 3/PRSS3 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002). The specific activity is >17,000 pmol/min/µg, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived mouse Trypsin 3/PRSS3 protein
Phe16-Asn246, with a C-terminal 10-His tag
Accession # NP_035775
Accession #
N-terminal Sequence
Structure / Form
Pro form
Predicted Molecular Mass
26 kDa
29 kDa and 36 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Supplied as a 0.2 μm filtered solution in HCl and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Assay Procedure

  • Activation Buffer: 50 mM Tris, 0.15 M NaCl, 10 mM CaCl2, 0.05% Brij-35 (w/v), pH 7.5 (TCNB)
  • Assay Buffer: 50 mM Tris, 0.15 M NaCl, 10 mM CaCl2, 0.05% Brij-35 (w/v), pH 8.0
  • Recombinant Mouse Trypsin 3/PRSS3 (rmTrypsin 3) (Catalog # 3565-SE)
  • Recombinant Human Enteropeptidase/Enterokinase (rhEnterokinase) (Catalog # 1585-SE)
  • Bacterial Thermolysin (Thermolysin) (Catalog # 3097-ZN)
  • 1,10 Phenanthroline (Sigma, Catalog # 320056) 0.6 M in DMSO
  • Substrate MCA-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2 (Catalog # ES002), 2 mM stock in DMSO
  • F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  • Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Activate rhEnterokinase with Thermolysin.
    1. Dilute rhEnterokinase to 100 µg/mL in Activation Buffer.
    2. Dilute Thermolysin to 3.16 µg/mL in Activation Buffer.
    3. Mix equal volumes of diluted rhEnterokinase and Thermolysin.
    4. Incubate at 37 °C for 30 minutes.
    5. Stop the reaction by adding an equal volume of 20 mM 1,10 Phenanthroline to the reaction tube.
  2. Activate rmTrypsin 3 with activated rhEnterokinase.
    1. Dilute activated rhEnterokinase to 2 µg/mL in Assay Buffer.
    2. Dilute rmTrypsin 3 to 200 µg/mL in Assay Buffer.
    3. Mix equal volumes of activated rhEnterokinase and rmTrypsin 3.
    4. Incubate at room temperature for 1 hour.
  3. Dilute activated rmTrypsin3 to 0.04 µg/mL in Assay Buffer.
  4. Dilute Substrate to 20 µM in Assay Buffer.
  5. Load 50 µL of 0.04 µg/mL of rhTrypsin 3 into a plate, and start the reaction by adding 50 µL of 20 µM Substrate. Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 20 µM Substrate.
  6. Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes.
  7. Calculate specific activity: 

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).

Per Well:
  • rmTrypsin 3: 0.002 µg
  • Substrate: 10 µM
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: Trypsin 3/PRSS3

Mouse Trypsin-3, encoded by the PRSS3 gene, is also known as mesotrypsin. It consists of a signal peptide (residues 1 to 15), a pro region (residues 16 to 23), and a mature chain (residues 24 to 246) (1). The purified recombinant mouse Trypsin 3 corresponds to the pro form, which can be activated by enterokinase.

  1. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1483, Academic Press, San Diego.
Entrez Gene IDs
5646 (Human); 22073 (Mouse); 362347 (Rat)
Alternate Names
Brain trypsinogen; EC 3.4.21; EC; Mesotrypsin; mesotrypsinogen; MTG; pancreatic trypsinogen III; protease, serine, 3 (mesotrypsin); protease, serine, 3; protease, serine, 4 (trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3MTG; TRY4mesotrypsin; Trypsin 3; Trypsin 4; Trypsin III; Trypsin IV; trypsin-3; Trypsinogen 15; trypsinogen 4; trypsinogen 5; trypsinogen IV


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