Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
643-VM-050
643-VM-200

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Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to mouse VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% will adhere after 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse VCAM-1/CD106 protein
Met Mouse VCAM-1
(Phe25-Glu698)
Accession # CAA47989
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
101.9 kDa (monomer)
SDS-PAGE
120 kDa, reducing conditions

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643-VM

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

643-VM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in sterile, deionized water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: VCAM-1/CD106

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.

During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.

ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Long Name
Vascular Cell Adhesion Molecule 1
Entrez Gene IDs
7412 (Human); 22329 (Mouse)
Alternate Names
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1

Citations for Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

48 Citations: Showing 1 - 10
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  1. Trogocytic molting of T cell microvilli upregulates T cell receptor surface expression and promotes clonal expansion
    Authors: Park, JS;Kim, JH;Soh, WC;Kim, NY;Lee, KS;Kim, CH;Chung, IJ;Lee, S;Kim, HR;Jun, CD;
    Nature communications
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells
    Authors: YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra
    Science Advances, 2022-08-24;8(34):eabn5522.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  3. ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice
    Authors: S Sevilla-Mo, P Fuentes, Y Rodríguez-, N Arellano-S, PW Krenn, SI de Val, S Montero-He, J García-Cec, V Burdiel-He, SR Gardeta, N Aguilera-M, C Barrio-Alo, G Crainiciuc, D Bouvard, A García-Par, AG Zapata, A Hidalgo, R Fässler, YR Carrasco, ML Toribio, J Teixidó
    European Journal of Immunology, 2022-05-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Thrombin-Activated Platelets Protect Vascular Endothelium against Tumor Cell Extravasation by Targeting Endothelial VCAM-1
    Authors: CM Lee, ML Chang, RH Chen, FW Chen, JC Liu, SL Kuo, HH Peng
    International Journal of Molecular Sciences, 2022-03-22;23(7):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  5. Plasma cell dynamics in the bone marrow niche
    Authors: Z Benet, Z Jing, DR Fooksman
    Cell Reports, 2021-02-09;34(6):108733.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. LFA-1 signals to promote actin polymerization and upstream migration in T cells
    Authors: NH Roy, SHJ Kim, A Buffone, D Blumenthal, B Huang, S Agarwal, PL Schwartzbe, DA Hammer, JK Burkhardt
    J. Cell. Sci., 2020-09-09;133(17):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo
    Authors: V Azcutia, M Kelm, AC Luissint, K Boerner, S Flemming, M Quiros, G Newton, A Nusrat, FW Luscinskas, CA Parkos
    Mucosal Immunol, 2020-06-19;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance
    Authors: V Barbier, J Erbani, C Fiveash, JM Davies, J Tay, MR Tallack, J Lowe, JL Magnani, DR Pattabiram, AC Perkins, J Lisle, JEJ Rasko, JP Levesque, IG Winkler
    Nat Commun, 2020-04-27;11(1):2042.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. beta1-integrin- and KV1.3 channel-dependent signaling stimulates glutamate release from Th17 cells
    Authors: K Birkner, B Wasser, T Ruck, C Thalman, D Luchtman, K Pape, S Schmaul, L Bitar, EM Krämer-Alb, A Stroh, SG Meuth, F Zipp, S Bittner
    J. Clin. Invest., 2020-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  10. Integrin CD11b activation drives anti-tumor innate immunity
    Authors: MC Schmid, SQ Khan, MM Kaneda, P Pathria, R Shepard, TL Louis, S Anand, G Woo, C Leem, MH Faridi, T Geraghty, A Rajagopala, S Gupta, M Ahmed, RI Vazquez-Pa, DA Cheresh, V Gupta, JA Varner
    Nat Commun, 2018-12-19;9(1):5379.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Methyltransferase Nsd2 Ensures Germinal Center Selection by Promoting Adhesive Interactions between B Cells and Follicular Dendritic Cells
    Authors: J Chen, N Li, Y Yin, N Zheng, M Min, B Lin, L Zhang, X Long, Y Zhang, Z Cai, S Zhai, J Qin, X Wang
    Cell Rep, 2018-12-18;25(12):3393-3404.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Cochaperone Mzb1 is a key effector of Blimp1 in plasma cell differentiation and ?1-integrin function
    Authors: V Andreani, S Ramamoorth, A Pandey, E Lupar, SL Nutt, T Lämmermann, R Grosschedl
    Proc. Natl. Acad. Sci. U.S.A., 2018-09-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus
    Authors: FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser
    Elife, 2018-09-06;7(0):.
    Species: Mouse
    Sample Types: Whole Cells
  14. Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis
    Authors: A Farinazzo, S Angiari, E Turano, E Bistaffa, S Dusi, S Ruggieri, R Bonafede, R Mariotti, G Constantin, B Bonetti
    Sci Rep, 2018-05-10;8(1):7473.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay
  15. RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice
    Authors: K Morita, C Tokushige, S Maeda, H Kiyose, M Noura, A Iwai, M Yamada, G Kashiwazak, J Taniguchi, T Bando, M Hirata, TR Kataoka, T Nakahata, S Adachi, H Sugiyama, Y Kamikubo
    Blood Adv, 2018-03-13;2(5):509-515.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Plasminogen Activator Inhibitor-1 Promotes Neutrophil Infiltration and Tissue Injury on Ischemia-Reperfusion
    Authors: M Praetner, G Zuchtriege, M Holzer, B Uhl, J Schaubäche, L Mittmann, M Fabritius, R Fürst, S Zahler, D Funken, M Lerchenber, A Khandoga, S Kanse, K Lauber, F Krombach, CA Reichel
    Arterioscler. Thromb. Vasc. Biol., 2018-01-25;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Kindlin-1 promotes pulmonary breast cancer metastasis
    Authors: S Sarvi, H Patel, J Li, GL Dodd, H Creedon, M Muir, J Ward, JC Dawson, M Lee, J Culley, DM Salter, AH Sims, A Byron, VG Brunton
    Cancer Res., 2018-01-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia
    Authors: S Paetau, T Rolova, L Ning, CG Gahmberg
    Front Mol Neurosci, 2017-12-22;10(0):431.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. PDE8 controls CD4(+) T cell motility through the PDE8A-Raf-1 kinase signaling complex
    Authors: CP Basole, RK Nguyen, K Lamothe, A Vang, R Clark, GS Baillie, PM Epstein, S Brocke
    Cell. Signal., 2017-08-26;40(0):62-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate
    Authors: PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley
    J. Leukoc. Biol., 2017-06-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  21. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
    Authors: KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis
    Nat. Immunol., 2017-04-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1
    Authors: S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra
    Nat. Methods, 2017-04-10;14(5):531-538.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Ena/VASP proteins regulate activated T-cell trafficking by promoting diapedesis during transendothelial migration
    Authors: ML Estin, SB Thompson, B Traxinger, MH Fisher, RS Friedman, J Jacobelli
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. MFG-E8-derived peptide attenuates adhesion and migration of immune cells to endothelial cells
    Authors: Y Hirano, WL Yang, M Aziz, F Zhang, B Sherry, P Wang
    J. Leukoc. Biol, 2017-01-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties
    Sci Rep, 2016-11-29;6(0):37914.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Aged neutrophils contribute to the first line of defense in the acute inflammatory response
    Blood, 2016-09-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. A Critical Role for P2X7 Receptor-Induced VCAM-1 Shedding and Neutrophil Infiltration during Acute Lung Injury
    J Immunol, 2016-08-24;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Post-arrest stalling rather than crawling favors CD8(+) over CD4(+) T-cell migration across the blood-brain barrier under flow in vitro
    Authors: Henriette Rudolph
    Eur J Immunol, 2016-07-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Platelets Guide Leukocytes to Their Sites of Extravasation
    PLoS Biol, 2016-05-06;14(5):e1002459.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the alpha4beta1-integrin.
    Authors: Martin-Blondel G, Pignolet B, Tietz S, Yshii L, Gebauer C, Perinat T, Van Weddingen I, Blatti C, Engelhardt B, Liblau R
    Eur J Immunol, 2015-10-06;45(12):3302-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Arsenic Exposure Increases Monocyte Adhesion to the Vascular Endothelium, a Pro-Atherogenic Mechanism.
    Authors: Lemaire M, Negro Silva L, Lemarie C, Bolt A, Flores Molina M, Krohn R, Smits J, Lehoux S, Mann K
    PLoS ONE, 2015-09-02;10(9):e0136592.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  32. B Lymphocyte-Specific Loss of Ric-8A Results in a Galpha Protein Deficit and Severe Humoral Immunodeficiency.
    Authors: Boularan C, Hwang I, Kamenyeva O, Park C, Harrison K, Huang Z, Kehrl J
    J Immunol, 2015-07-31;195(5):2090-102.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Toll-like receptor 4 and MAIR-II/CLM-4/LMIR2 immunoreceptor regulate VLA-4-mediated inflammatory monocyte migration.
    Authors: Totsuka N, Kim Y, Kanemaru K, Niizuma K, Umemoto E, Nagai K, Tahara-Hanaoka S, Nakahasi-Oda C, Honda S, Miyasaka M, Shibuya K, Shibuya A
    Nat Commun, 2014-08-19;5(0):4710.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  34. Cytoskeletal regulatory gene expression and migratory properties of B-cell progenitors are affected by the ETV6-RUNX1 rearrangement.
    Authors: Palmi C, Fazio G, Savino A, Procter J, Howell L, Cazzaniga V, Vieri M, Longinotti G, Brunati I, Andre V, Della Mina P, Villa A, Greaves M, Biondi A, D'Amico G, Ford A, Cazzaniga G
    Mol Cancer Res, 2014-07-24;12(12):1796-806.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Phospholipase D1 mediates lymphocyte adhesion and migration in experimental autoimmune encephalomyelitis.
    Authors: Gobel K, Schuhmann M, Pankratz S, Stegner D, Herrmann A, Braun A, Breuer J, Bittner S, Ruck T, Wiendl H, Kleinschnitz C, Nieswandt B, Meuth S
    Eur J Immunol, 2014-06-05;44(8):2295-305.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness.
    Authors: Wang X, Rodda L, Bannard O, Cyster J
    J Immunol, 2014-04-16;192(10):4601-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  37. Endothelial cell-derived chemerin promotes dendritic cell transmigration.
    Authors: Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S
    J Immunol, 2014-01-27;192(5):2366-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  38. Characterization of VCAM-1-binding peptide-functionalized quantum dots for molecular imaging of inflamed endothelium.
    Authors: Chen Y, Molnar M, Li L, Friberg P, Gan L, Brismar H, Fu Y
    PLoS ONE, 2013-12-31;8(12):e83805.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  39. A circadian clock gene, Rev-erbalpha, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression.
    Authors: Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H, Kizaki T
    J Immunol, 2013-12-04;192(1):407-17.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  40. A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling.
    Authors: Lee J, Sohn J, Ryu S, Hong C, Moon K, Park J
    J Cell Mol Med, 2013-07-16;17(10):1271-81.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  41. PI3-kinase gamma promotes Rap1a-mediated activation of myeloid cell integrin alpha4beta1, leading to tumor inflammation and growth.
    Authors: Schmid M, Franco I, Kang S, Hirsch E, Quilliam L, Varner J
    PLoS ONE, 2013-04-02;8(4):e60226.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  42. Toxoplasma gondii modulates the dynamics of human monocyte adhesion to vascular endothelium under fluidic shear stress.
    Authors: Harker K, Ueno N, Wang T, Bonhomme C, Liu W, Lodoen M
    J Leukoc Biol, 2013-03-13;93(5):789-800.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. Difference in Th1 and Th17 lymphocyte adhesion to endothelium.
    Authors: Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH
    J. Immunol., 2012-01-04;188(3):1421-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  44. Kindlin-3 is required for beta2 integrin-mediated leukocyte adhesion to endothelial cells.
    Authors: Moser M, Bauer M, Schmid S, Ruppert R, Schmidt S, Sixt M, Wang HV, Sperandio M, Fassler R
    Nat. Med., 2009-02-22;15(3):300-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  45. IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit.
    Authors: Sasaki K, Pardee AD, Okada H, Storkus WJ
    Eur. J. Immunol., 2008-10-01;38(10):2865-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  46. High pressure promotes monocyte adhesion to the vascular wall.
    Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S
    Circ. Res., 2007-03-29;100(8):1226-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  47. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
    Authors: Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
    J. Leukoc. Biol., 2006-12-18;81(3):748-56.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  48. CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin.
    Authors: Cardones AR, Murakami T, Hwang ST
    Cancer Res., 2003-10-15;63(20):6751-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry

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