Angiotensin I Converting Enzyme 2 (ACE-2), also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). The predicted rat ACE-2 protein sequence consists of 805 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves Angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of cardiovascular function in vivo (3). In addition, ACE-2 has a critical function in acute lung injury (4).
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Key Product Details
- R&D Systems NS0-derived Recombinant Rat ACE-2 Protein (4516-ZN)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived rat ACE-2 protein
Met1-Thr740, with a C-terminal 10-His tag
Met1-Thr740, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No results obtained: Gln18 predicted
Predicted Molecular Mass
85 kDa
SDS-PAGE
120 kDa, reducing conditions
Activity
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007).
The specific activity is >300 pmol/min/µg, as measured under the described conditions.
The specific activity is >300 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
4516-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ACE-2
References
- Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238.
- Crackower, M.A. et al. (2002) Nature 417:822.
- Huang, L. et al. (2003) J. Biol. Chem. 278:15532.
- Kuba, K. et al. (2006) Curr. Opinion Pharmacol. 6:271.
Long Name
Angiotensin I Converting Enzyme 2
Alternate Names
ACE2, ACEH
Entrez Gene IDs
Gene Symbol
ACE2
UniProt
Additional ACE-2 Products
Product Documents for Recombinant Rat ACE-2 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Rat ACE-2 Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Rat ACE-2 Protein, CF (4516-ZN):
Materials
- Assay Buffer: 50 mM Tris, 1 M NaCl, pH 7.5
- Recombinant Rat ACE-2 (rrACE-2) (Catalog # 4516-ZN)
- Substrate: MCA-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH (Catalog # ES007), 2 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rrACE-2 to 0.5 ng/μL in Assay Buffer.
- Dilute Substrate 20 μM in Assay Buffer.
- In plate load 50 μL of 0.5 ng/μL rrACE-2. In a separate well load 50 µL Assay Buffer to be used as a Substrate Blank.
- Start the reaction by adding 50 μL of 20 μM Substrate to all wells.
- Read at excitation and emission wavelengths of 320 nm and 405 nm (top read), respectively in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard MCA-Pro-Leu-OH (Bachem, Catalog # M-1975).
Per Well:
- rrACE-2: 0.025 µg
- Substrate: 10 µM
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Associated Pathways
A-beta Pathways: Uptake & Degradation
