Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

Substrate for ACE-2 and Caspase-1
Catalog # Availability Size / Price Qty
ES007
Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (1)

Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate Summary

Purity
>95%, by HPLC.
Activity
The peptide substrate contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence. It is an excellent substrate for caspase-1/interleukin-converting enzyme (ICE) and angiotensin I-converting enzyme-2 (ACE-2) (Enari, M. et al., 1996, Nature 380:723; Vickers, C. et al., 2002, J. Biol. Chem. 277:14838). The cleavage sites by ICE and ACE-2 are the peptide bonds between Ala and Asp and between Pro and Lys, respectively.
Source

Mca-Tyr-Val-Ala-Asp-Ala-Pro-Lys(Dnp)-OH.
Mca: (7-Methoxycoumarin-4-yl)acetyl, Dnp: 2, 4-Dinitrophenyl.
Predicted Molecular Mass
1145.2 Da

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

ES007

Formulation Supplied as a stock solution in dimethyl sulfoxide (DMSO) at a concentration of 6.19 mg/mL or 4.0 mM.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: No Stability Data Storage is set to N/A for ProdCode
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Citations for Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA
    Authors: E Schrom, M Huber, M Aneja, C Dohmen, D Emrich, J Geiger, G Hasenpusch, A Herrmann-J, V Kretzschma, O Mykhailyk, T Pasewald, P Oak, A Hilgendorf, D Wohlleber, HG Hoymann, D Schaudien, C Plank, C Rudolph, R Kubisch-Do
    Mol Ther Nucleic Acids, 2017;7(0):350-365.
    Species: Human
    Sample Types: Tissue Homogenates
  2. Circulating levels of tumor necrosis factor-alpha receptor 2 are increased in heart failure with preserved ejection fraction relative to heart failure with reduced ejection fraction: evidence for a divergence in pathophysiology.
    Authors: Putko B, Wang Z, Lo J, Anderson T, Becher H, Dyck J, Kassiri Z, Oudit G
    PLoS ONE, 2014;9(6):e99495.
    Species: Human
    Sample Types: Plasma
    Applications: Enzyme Assay
  3. Angiotensin-converting enzyme 2 (ACE2) activator diminazene aceturate ameliorates endotoxin-induced uveitis in mice.
    Authors: Qiu Y, Shil P, Zhu P, Yang H, Verma A, Lei B, Li Q
    Invest Ophthalmol Vis Sci, 2014;55(6):3809-18.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Enzyme Assay
  4. ACE2 is augmented in dystrophic skeletal muscle and plays a role in decreasing associated fibrosis.
    Authors: Riquelme C, Acuna M, Torrejon J, Rebolledo D, Cabrera D, Santos R, Brandan E
    PLoS ONE, 2014;9(4):e93449.
    Species: Mouse
    Sample Types: Tissue Homogenates
  5. Dicer expression exhibits a tissue-specific diurnal pattern that is lost during aging and in diabetes.
    Authors: Yan, Yuanqing, Salazar, Tatiana, Dominguez, James M, Nguyen, Dung V, Li Calzi, Sergio, Bhatwadekar, Ashay D, Qi, Xiaoping, Busik, Julia V, Boulton, Michael, Grant, Maria B
    PLoS ONE, 2013;8(11):e80029.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay
  6. Sex differences in angiotensin-converting enzyme modulation of Ang (1-7) levels in normotensive WKY rats.
    Authors: Bhatia, Kanchan, Zimmerman, Margaret, Sullivan, Jennifer
    Am J Hypertens, 2013;26(5):591-8.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  7. Astacin proteases cleave dentin sialophosphoprotein (Dspp) to generate dentin phosphoprotein (Dpp).
    Authors: Tsuchiya S, Simmer JP, Hu JC, Richardson AS, Yamakoshi F, Yamakoshi Y
    J. Bone Miner. Res., 2010;26(0):220.
    Species: Human
    Sample Types:
    Applications: Bioassay
  8. Major role for ACE-independent intrarenal ANG II formation in type II diabetes.
    Authors: Park S, Bivona BJ, Kobori H, Seth DM, Chappell MC, Lazartigues E, Harrison-Bernard LM
    Am. J. Physiol. Renal Physiol., 2010;298(1):F37-48.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Enzyme Assay
  9. Activation of the epidermal growth factor receptor (EGFR) by a novel metalloprotease pathway.
    Authors: Bergin DA, Greene CM, Sterchi EE, Kenna C, Geraghty P, Belaaouaj A, Taggart CC, O'Neill SJ, McElvaney NG
    J. Biol. Chem., 2008;283(46):31736-44.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Enzyme Assay Substrate
  10. Angiotensin converting enzyme-2 confers endothelial protection and attenuates atherosclerosis.
    Authors: Lovren F, Pan Y, Quan A, Teoh H, Wang G, Shukla PC, Levitt KS, Oudit GY, Al-Omran M, Stewart DJ, Slutsky AS, Peterson MD, Backx PH, Penninger JM, Verma S
    Am. J. Physiol. Heart Circ. Physiol., 2008;295(4):H1377-84.
    Species: Human
    Sample Types: Cell Culture Supernates

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Mca-YVADAPK(Dnp)-OH Fluorogenic Peptide Substrate
By Anonymous on 08/04/2016
Application:

ACE-2 activity was determined following incubation with intramolecularly quenched synthetic ACE-2 specific substrate 7-Mca-YVADAPK (Dnp) (R&D systems). In the case of cell lysates, 10 µg of total protein was assayed for activity in a buffer with the following composition: 50 mM MES (4-morpholineethanesulphonic acid), 300 mM NaCl, 10 µm ZnCl2 and 0.01% Triton X-100, pH 6.5. Reaction was initiated by the addition of 5×10−5 M substrate. Where applicable, recombinant enzymes were used at a concentration of 0.01 µg per reaction. The fluorescence measurements were performed in the black microtiter plates (Costar) in a total volume of 100 µl. The plates were read using a fluorescence plate reader SpectraMax M5 (Molecular Devices) at an excitation wavelength 320 nm and emission wavelength 405 nm Fluorescence resulting from the substrate hydrolysis increased with time, and achieved maximum by one h with recombinant enzyme.