Recombinant Rat IL-1 alpha/IL-1F1 Protein

Formulations:
Catalog # Availability Size / Price Qty
500-RL-005
500-RL-025
Product Details
Citations (5)
FAQs
Reviews (1)

Recombinant Rat IL-1 alpha/IL-1F1 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 1-7 pg/mL.
Source
E. coli-derived rat IL-1 alpha/IL-1F1 protein
Ser115-Ser270
Accession #
N-terminal Sequence
Analysis
Ser115
Predicted Molecular Mass
18 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

500-RL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

500-RL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid (aa) sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporally upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the aa sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

References
  1. Dower, S.K. and J. Z. Sims, (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A., ed., Oxford University Press, New York p. 17.
Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

Citations for Recombinant Rat IL-1 alpha/IL-1F1 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. BST106 protects against cartilage damage by inhibition of apoptosis and enhancement of autophagy in osteoarthritic rats
    Authors: JM Hong, JK Shin, JY Kim, MJ Jang, SK Park, JH Lee, JH Choi, SM Lee
    Biol. Pharm. Bull., 2018;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  2. NG2 glial cells regulate neuroimmunological responses to maintain neuronal function and survival
    Authors: M Nakano, Y Tamura, M Yamato, S Kume, A Eguchi, K Takata, Y Watanabe, Y Kataoka
    Sci Rep, 2017;7(0):42041.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  3. Interleukin 1 alpha (IL1A) is a novel regulator of the blood-testis barrier in the rat.
    Authors: Sarkar O, Mathur PP, Cheng CY, Mruk DD
    Biol. Reprod., 2007;78(3):445-54.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  4. Alpha-4/beta-1 and alpha-L/beta-2 integrins mediate cytokine induced lung leukocyte-epithelial adhesion and injury.
    Authors: Parmley LA, Elkins ND, Fini MA, Liu YE, Repine JE, Wright RM
    Br. J. Pharmacol., 2007;152(6):915-29.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  5. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006;140(1):87-100.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Rat IL-1 alpha/IL-1F1 Protein

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Recombinant Rat IL-1 alpha/IL-1F1 Protein
By Anonymous on 11/07/2017
Application: Immunoassay Standard

The recombinant rat IL-1a was used as an ELISA standard. The ELISA was used to measure serum levels of IL-1a.