IL-13 is a 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergic asthma and atopy. It is secreted by Th1 and Th2 CD4+ T cells, NK cells, visceral smooth muscle cells, eosinophils, mast cells, and basophils (1 - 3). IL-13 circulates as a monomer with two internal disulfide bonds that contribute to a bundled four alpha -helix configuration (4, 5). Mature rat IL-13 shares 59%, 75%, and 60% amino acid sequence identity with human, mouse, and rhesus IL-13, respectively. Despite the low homology, it exhibits cross-species activity between human, mouse, and rat (6, 7). IL-13 has diverse activities on numerous cell types (8). On macrophages, IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances. On B cells, IL-13 induces immunoglobulin class switching to IgE, upregulates the expression of MHC class II, CD71, CD72, and CD23, and costimulates proliferation. IL-13 upregulates IL-6 while downregulating IL-1 and TNF-alpha production by fibroblasts and endothelial cells. IL-13 binds with low affinity to IL-13 R alpha 1, triggering IL-13 R alpha 1 association with IL-4 R alpha. This high affinity receptor complex also functions as the type 2 IL-4 receptor complex (9, 10). Additionally, IL-13 binds with high affinity to IL-13 R alpha 2 which is expressed intracellularly, on the cell surface, and as a soluble molecule (11 - 14). IL-13 R alpha 2 regulates the bioavailability of both IL-13 and IL-4 and is overexpressed in glioma and several bronchial pathologies (10, 15, 16). Compared to wild type IL-13, the atopy-associated R110Q variant of IL-13 elicits increased responsiveness from eosinophils that express low levels of IL-13 R alpha 2 (17).
Key Product Details
- R&D Systems E. coli-derived Recombinant Rat IL-13 Protein (1945-RL)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Thr19-His131
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 1-4 ng/mL.
Reviewed Applications
Read 2 reviews rated 4.5 using 1945-RL in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1945-RL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1945-RL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: IL-13
References
- Wills-Karp, M. (2004) Immunol. Rev. 202:175.
- Nakajima H. and K. Takatsu (2007) Int. Arch. Allergy Immunol. 142:265.
- Lakkis, F.G and E.N. Cruet (1993) Biochem. Biophys. Res. Commun. 197:612.
- Moy, F.J. et al. (2001) J. Mol. Biol. 310:219.
- Eisenmesser, E.Z. et al. (2001) J. Mol. Biol. 310:231.
- Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
- Lakkis, F.G. et al. (1997) Biochem. Biophys. Res. Commun. 235:529.
- Wynn, T.A. (2003) Annu. Rev. Immunol. 21:425.
- Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
- Tabata, Y. et al. (2007) Curr. Allergy Asthma Rep. 7:338.
- Chiaramonte, M.G. et al. (2003) J. Exp. Med. 197:687.
- Daines, M.O. and G.K. Hershey (2002) J. Biol. Chem. 227:10387
- Matsumura, M. et al. (2007) Biochem. Biophys. Res. Commun.360:464.
- Tabata, Y. et al. (2007) J. Immunol. 177:7905.
- Andrews, A.L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
- Joshi, B.H. et al. (2006) Vitam. Horm. 74:479.
- Andrews, A.-L. et al. (2007) J. Allergy Clin. Immunol. 120:91.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional IL-13 Products
Product Documents for Recombinant Rat IL-13 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Rat IL-13 Protein
For research use only
Citations for Recombinant Rat IL-13 Protein
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Customer Reviews for Recombinant Rat IL-13 Protein (2)
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/20/2021
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Application: Enzymatic activity in vitroVerified Customer | Posted 01/15/2016Added IL-13 10ng/mL and 100ng/mL to full culture media overnight and measured secreted reporter the next day.
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