Recombinant Rat Notch-2 Fc Chimera Protein, CF

  
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  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. Immobilized rrNotch-2/Fc Chimera can bind rrJagged-1/Fc Chimera with an apparent KD <5 nM.
  • Source
    Mouse myeloma cell line, NS0-derived
    Rat Notch-2
    (Leu26 - Glu492)
    Accession # Q9QW30
    IEGRMD Human IgG1
    (Pro100 - Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu26
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    76.5 kDa (monomer)
  • SDS-PAGE
    105-115 kDa, reducing conditions
1190-NT
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Notch-2

Rat Notch-2 is a 300 kDa, type I transmembrane glycoprotein involved in a number of early-event developmental processes (1). In both vertebrates and invertebrates, Notch signaling is important for specifying cell fates and for defining boundaries between different cell types. The molecule is synthesized as a 2472 amino acid (aa) precursor that contains a putative 27 aa signal sequence, a 1650 aa extracellular region, a 23 aa transmembrane (TM) segment and a 772 aa cytoplasmic domain (2). The large Notch extracellular domain has 36 EGF-like repeats followed by three notch/Lin-12 repeats (LNR). Of the 36 EGF-like repeats, the 11th and 12thEGF-like repeats have been shown to be both necessary and sufficient for binding the ligands Serrate and Delta, in Drosophila (3). Cell surface Notch receptor is thought to be a heterodimer consisting of the ligand binding extracellular region associated with the remaining transmembrane protein, as a result of post-translational proteolytic cleavage by a furin-like enzyme. Upon ligand binding, additional proteolysis events result in the release of the Notch intracellular domain (NICD). NICD translocates into the nucleus and initiates transcription of Notch-responsive genes (4). Thus Notch acts as both a ligand-binding receptor and a nuclear factor that regulates transcription. In addition, an alternative Notch signaling pathway that is mediated by the full-length, uncleaved form of Notch-1 at the cell surface has been reported to suppress differentiation of myoblasts in response to ligand binding (5). Rat Notch-2 shows 92% and 95% aa identity to human and mouse Notch-2 extracellular domains, respectively. Relative to the extracellular region of rat Notch-1, rat Notch-2 exhibits 56% aa identity.

  • References:
    1. Weinmaster, G. (2000) Curr. Opin. Genet. Dev. 10:363.
    2. Weinmaster, G. (1992) Development 116:931.
    3. Rebay, I. et al. (1991) Cell 67:687.
    4. Mumm, J.S. and R. Kopan (2000) Dev. Biol. 228:151.
    5. Bush, G. et al. (2001) Dev. Biol. 229:494.
  • Entrez Gene IDs:
    4853 (Human); 29492 (Rat)
  • Alternate Names:
    AGS2; hN2; neurogenic locus notch homolog protein 2; Notch (Drosophila) homolog 2; notch 2Notch homolog 2 (Drosophila); Notch homolog 2; Notch2; Notch-2
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