![Western Blot: RECQL5 Antibody [NBP1-40341]](https://resources.rndsystems.com/images/products/RECQL5-Antibody-Western-Blot-NBP1-40341-img0002.jpg "Western Blot: RECQL5 Antibody [NBP1-40341]")
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Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 650 and 700 of human RecQ protein-like 5 using the numbering given in entry NP_004250.4 (GeneID 9400).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RECQL5 Antibody
Western Blot: RECQL5 Antibody [NBP1-40341]
Western Blot: RECQL5 Antibody [NBP1-40341] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RECQ5 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. RECQ5 was also immunoprecipitated by rabbit anti-RECQ5 antibodies NBP1-40339 and NBP1-40340which recognize upstream epitopes.Applications for RECQL5 Antibody
Application
Recommended Usage
Immunoprecipitation
5-15 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RECQL5
Alternate Names
DNA helicase, RecQ-like type 5, EC 3.6.1, EC 3.6.4.12, FLJ90603, RecQ protein-like 5ATP-dependent DNA helicase Q5, RecQ5, RECQ5RecQ protein 5
Entrez Gene IDs
9400 (Human)
Gene Symbol
RECQL5
Additional RECQL5 Products
Product Documents for RECQL5 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RECQL5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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