RelA/NFkB p65 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-56502
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Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human
Predicted:
Canine (93%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids within 1 and 50 of human p65 protein was used as immunogen.
Reactivity Notes
Reacts with Chimpanzee and New World Monkey. Predicted to react with Canine, Mouse and Rat.
Specificity
The amino acid sequence used as immunogen is 100% homologous in human (isoform CRA_a, CRA_c)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit RelA/NFkB p65 Antibody - BSA Free (NB100-56502) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-RelA/NFkB p65 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RelA/NFkB p65 Antibody - BSA Free
Western Blot: RelA/NFkB p65 Antibody [NB100-56502]
Western Blot: RelA/NFkB p65 Antibody [NB100-56502] - Analysis of extracts from various cell lines using NFkB p65 antibody at 1:100 (5 ug/mL).Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 Antibody [NB100-56502]
Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 Antibody [NB100-56502] - p65 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Western Blot: RelA/NFkB p65 Antibody [NB100-56502]
Western Blot: RelA/NFkB p65 Antibody [NB100-56502] - Analysis of p65 in HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody.Applications for RelA/NFkB p65 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/mL
Western Blot
1:100 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 0.2% gelatin
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RelA/NFkB p65
Long Name
v-rel Reticuloendotheliosis Viral Oncogene Homolog A
Alternate Names
NFkB p65, NFKB3, p65RelA
Entrez Gene IDs
5970 (Human)
Gene Symbol
RELA
UniProt
Additional RelA/NFkB p65 Products
Product Documents for RelA/NFkB p65 Antibody - BSA Free
Product Specific Notices for RelA/NFkB p65 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for RelA/NFkB p65 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars