RENT1/UPF1/hUPF1 Antibody (5D5C4)

Name: RENT1/UPF1/hUPF1 Antibody (5D5C4)
Brand: Novus Biologicals
SKU: NBP3-16544
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16544

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5D5C4 expressed in HEK293

View all RENT1/UPF1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RENT1/UPF1/hUPF1 (Q92900). MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RENT1/UPF1/hUPF1 Antibody (5D5C4)

Western Blot: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Western Blot: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544] - Western blot analysis of extracts of various cell lines, using RENT1/UPF1/hUPF1 Rabbit mAb (NBP3-16544) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Immunocytochemistry/Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544] - Immunofluorescence analysis of C6 cells using RENT1/UPF1/hUPF1 Rabbit mAb (NBP3-16544) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544]

Immunoprecipitation: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug RENT1/UPF1/hUPF1 antibody (NBP3-16544). Western blot was performed from the immunoprecipitate using RENT1/UPF1/hUPF1 antibody (NBP3-16544) at a dilition of 1:1000.

Immunocytochemistry/ Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544] -

Immunocytochemistry/ Immunofluorescence: RENT1/UPF1/hUPF1 Antibody (5D5C4) [NBP3-16544] - Confocal imaging of C6 cells using RENT1/UPF1/hUPF1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

View 1 more images

Applications for RENT1/UPF1/hUPF1 Antibody (5D5C4)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RENT1/UPF1

hUPF1 encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq]

Long Name

Regulator of Nonsense Transcripts 1

Alternate Names

NORF1, UPF1

Gene Symbol

UPF1

Additional RENT1/UPF1 Products

Product Documents for RENT1/UPF1/hUPF1 Antibody (5D5C4)

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RENT1/UPF1/hUPF1 Antibody (5D5C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RENT1/UPF1/hUPF1 Antibody (5D5C4)

There are currently no reviews for this product. Be the first to review RENT1/UPF1/hUPF1 Antibody (5D5C4) and earn rewards!

Have you used RENT1/UPF1/hUPF1 Antibody (5D5C4)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image