RFWD3 Antibody

Novus Biologicals | Catalog # NB100-68208

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Monkey (100%), Porcine (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RFWD3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 724 and 774 of human ring finger and WD repeat domain 3 using the numbering given in entry NP_060594.3 (GeneID 55159).

Reactivity Notes

Orangutan, Monkey, Gorilla and Chimpanzee (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFWD3 Antibody

Western Blot: RFWD3 Antibody [NB100-68208]

Western Blot: RFWD3 Antibody [NB100-68208]

Western Blot: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFWD3 antibody NB100-68208 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RFWD3 Antibody [NB100-68208]

Immunoprecipitation: RFWD3 Antibody [NB100-68208]

Immunoprecipitation: RFWD3 Antibody [NB100-68208] - Detection of human RFWD3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: RFWD3 was immunoprecipitated by rabbit anti-RFWD3 antibodies NB100-68206 and NB100-68207. For blotting immunoprecipitated RFWD3, NB100-68208 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for RFWD3 Antibody

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
Immunoprecipitation: Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RFWD3

RFWD3 (ring finger and WD repeat domain 3) has been identified as a protein that is phosphorylated in response to DNA damage. The exact function of RFWD3 (ring finger and WD repeat domain 3) has not been characterized. Structurally, RFWD3 possesses a RING-type zinc finger and 3 WD repeats.

Alternate Names

EC 6.3.2.-, FLJ10520, ring finger and WD repeat domain 3, RING finger and WD repeat domain-containing protein 3, RING finger protein 201, RNF201E3 ubiquitin-protein ligase RFWD3

Entrez Gene IDs

55159 (Human)

Gene Symbol

RFWD3

UniProt

NP_060594.3 (Human)

Additional RFWD3 Products

Product Documents for RFWD3 Antibody

Certificate of Analysis

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Product Specific Notices for RFWD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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