RFX6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48677

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RFX6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SHCSTYPEPIYPTLPQANHDFYSTSSNYQTVFRAQPHSTSGLYPHHTEHGRCMAWTEQQLSRDFFSGSCAGSPYNSRPPSSYGPSLQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RFX6 Antibody - BSA Free

Western Blot: RFX6 Antibody [NBP2-48677]

Western Blot: RFX6 Antibody [NBP2-48677]

Western Blot: RFX6 Antibody [NBP2-48677] - Analysis in control (vector only transfected HEK293T lysate) and RFX6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677]

Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677]

Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.
Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677]

Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677]

Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677] - Staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677]

Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677]

Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677] - Staining of human fallopian tube shows weak positivity in cilia in glandular cells.
Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677]

Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677]

Immunohistochemistry-Paraffin: RFX6 Antibody [NBP2-48677] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
RFX6 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: RFX6 Antibody - BSA Free [NBP2-48677]

Chromatin Immunoprecipitation-exo-Seq: RFX6 Antibody - BSA Free [NBP2-48677]

ChIP-Exo-Seq composite graph for Anti-RFX6 (NBP2-48677) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for RFX6 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RFX6

RFX6 is a member of the regulatory factor X (RFX) family of transcription factors (see RFX1; MIM 600006) (Aftab et al., 2008 [PubMed 18673564]).[supplied by OMIM]

Long Name

Regulatory Factor X, 6

Alternate Names

MTFS, RFXDC1

Gene Symbol

RFX6

Additional RFX6 Products

Product Documents for RFX6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RFX6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Diabetes

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Protocols

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FAQs

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