Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RGNEF Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1655 and 1705 of human 190 kDa, Rho-Guanine Nucleotide Exchange Factor using the numbering given in entry NP_001171164.1 (GeneID 64283).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RGNEF Antibody - BSA Free

Western Blot: RGNEF Antibody [NBP2-22282]

Western Blot: RGNEF Antibody [NBP2-22282]

Western Blot: RGNEF Antibody [NBP2-22282] - Whole cell lysate from HeLa (15 and 50 ug), 293T (50 ug), and Jurkat (50 ug) cells. NBP2-22282 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RGNEF Antibody [NBP2-22282]

Immunoprecipitation: RGNEF Antibody [NBP2-22282]

Immunoprecipitation: RGNEF Antibody [NBP2-22282] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RGNEF antibody NBP2-22282 used for IP at 6 ug/mg lysate. RGNEF was also immunoprecipitated by rabbit anti-RGNEF antibody. For blotting immunoprecipitated RGNEF, NBP2-22282 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for RGNEF Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RGNEF

Alternate Names

190 kDa guanine nucleotide exchange factor, p190RHOGEF, Rho-guanine nucleotide exchange factor

Gene Symbol

ARHGEF28

UniProt

NP_001171164.1 (Human)

Additional RGNEF Products

Product Documents for RGNEF Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RGNEF Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RGNEF Antibody - BSA Free

There are currently no reviews for this product. Be the first to review RGNEF Antibody - BSA Free and earn rewards!

Have you used RGNEF Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies