RGS4 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94071

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all RGS4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RGS4 (NP_005604.1). MCKGLAGLPASCLRSAKDMKHRLGFLLQKSDSCEHNSSHNKKDKVVICQRVSQEEVKKWAESLENLISHECGLAAFKAFLKSEYSEENIDFWISCEEYKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RGS4 Antibody - Azide and BSA Free

Western Blot: RGS4 AntibodyAzide and BSA Free [NBP2-94071]

Western Blot: RGS4 AntibodyAzide and BSA Free [NBP2-94071]

Western Blot: RGS4 Antibody [NBP2-94071] - Analysis of extracts of various cell lines, using RGS4 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: RGS4 Antibody - Azide and BSA Free [NBP2-94071]

Immunocytochemistry/ Immunofluorescence: RGS4 Antibody - Azide and BSA Free [NBP2-94071]

Immunocytochemistry/Immunofluorescence: RGS4 Antibody [NBP2-94071] - Analysis of U-2 OS cells using RGS4 at dilution of 1:100. Blue: DAPI for nuclear staining.
RGS4 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RGS4 Antibody - Azide and BSA Free [NBP2-94071] -

Immunocytochemistry/ Immunofluorescence: RGS4 Antibody - Azide and BSA Free [NBP2-94071] - Immunofluorescence analysis of L929 cells using RGS4 Rabbit pAb (A1787) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: RGS4 Antibody - Azide and BSA Free [NBP2-94071]

Immunocytochemistry/ Immunofluorescence: RGS4 Antibody - Azide and BSA Free [NBP2-94071]

Immunocytochemistry/Immunofluorescence: RGS4 Antibody [NBP2-94071] - Immunofluorescence analysis of C6 cells using RGS4 antibody (NBP2-94071) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for RGS4 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RGS4

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP bound forms. Regulator of G protein signaling 4 belongs to this family. All RGS proteins share a conserved 120 amino acid sequence termed the RGS domain. Regulator of G protein signaling 4 protein is 37% identical to RGS1 and 97% identical to rat RGS4. This protein negatively regulates signaling upstream or at the level of the heterotrimeric G protein and is localized in the cytoplasm.

Long Name

Regulator of G-protein signaling 4

Alternate Names

RGP4

Gene Symbol

RGS4

Additional RGS4 Products

Product Documents for RGS4 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RGS4 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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