Ribonuclease A Antibody [Biotin]

Name: Ribonuclease A Antibody [Biotin]
Brand: Novus Biologicals
SKU: NB100-2007
Price: 589 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-2007

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Bovine

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

View all Ribonuclease A Primary Antibodies »

Product Specifications

Immunogen

Ribonuclease A [Bovine pancreas] (Uniprot: P61823)

Reactivity Notes

Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined

Localization

Secreted

Specificity

Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ribonuclease A [Bovine Pancreas]

Scientific Data Images for Ribonuclease A Antibody [Biotin]

Western Blot: Ribonuclease A Antibody [Biotin] [NB100-2007] - Lane 1: Ribonuclease A (Bovine Pancreas). Load: 50 ng per lane. Primary antibody: Rabbit anti-Ribonuclease A Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 16.5 kDa, 16.5 kDa for Ribonuclease A. Other band(s): Possible dimerization.

Applications for Ribonuclease A Antibody [Biotin]

Application

Recommended Usage

ELISA

1:5000-1:20000

Immunoprecipitation

1:100

Western Blot

1:500-1:5000

Application Notes

This product has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Ribonuclease A in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:13,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Ribonuclease A

The Ribonuclease A gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substr

Alternate Names

EC 3.1.27.5, HP-RNase, MGC12408, RIB1, RIB-1, Ribonuclease 1, Ribonuclease A, ribonuclease pancreatic, ribonuclease, RNase A family, 1 (pancreatic), RNase A, RNase UpI-1, RNS1RNase 1

Gene Symbol

RNASE1

UniProt

P61823 (Bovine)

Additional Ribonuclease A Products

Product Documents for Ribonuclease A Antibody [Biotin]

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Certificate of Analysis

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Product Specific Notices for Ribonuclease A Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ribonuclease A Antibody [Biotin]

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