Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-13571
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%), Chicken (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT829
Format
Azide and BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser235 of human Ribosomal Protein S6/RPS6. The exact sequence is proprietary.
Modification
p Ser 235
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images
Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: Ribosomal Protein S6/RPS6 [p Ser235] Antibody (GT829) [NBP3-13571] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Ribosomal Protein S6/RPS6 (phospho Ser235) antibody [GT829] (NBP3-13571) diluted at 1:1000.
Applications
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ribosomal Protein S6/RPS6
Alternate Names
RPS6
Gene Symbol
RPS6
Additional Ribosomal Protein S6/RPS6 Products
Product Documents
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
