Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Alme-1

Format

BSA Free
View all RIG-I Primary Antibodies »

Product Specifications

Immunogen

Recombinant human RIG-I (aa 201-713).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RIG-I Antibody (Alme-1) - BSA Free

Western Blot: RIG-I Antibody (Alme-1)BSA Free [NBP2-80055]

Western Blot: RIG-I Antibody (Alme-1)BSA Free [NBP2-80055]

Western Blot: RIG-I Antibody (Alme-1) [NBP2-80055] - Analysis of human RIG-I in HeLa cells by using RIG-I, mAb (Alme-1). Cell extracts from HeLa cells either unstimulated (lane 1) or stimulated for 6h (lane 2), 16h (lane 3) or 24 h (lane 4) with Interferon gamma were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the RIG-I, mAb (Alme-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system.
Immunohistochemistry-Paraffin: RIG-I Antibody (Alme-1) - BSA Free [NBP2-80055]

Immunohistochemistry-Paraffin: RIG-I Antibody (Alme-1) - BSA Free [NBP2-80055]

Immunohistochemistry-Paraffin: RIG-I Antibody (Alme-1) [NBP2-80055] - Immunohistochemical staining of endogenous human RIG-I in different human tissues (paraffin sections) using anti-RIG-I, mAb (Alme-1). Different human normal tissues (A: Nasopharynx; B: Colon; C: Endometrium) or cancer tissues (D: Breast; E: Skin; F: Ovarian) were stained with anti-RIG-I, mAb (Alme-1) by standard immunohistochemistry.

Applications for RIG-I Antibody (Alme-1) - BSA Free

Application
Recommended Usage

Immunoprecipitation

1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 10% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIG-I

RIG-I, also known as DDX58, consists of a 106.6 kDa and a 101.4 kDa isoform and is involved in recognizing viruses and activating a response. Current research is being conducted with diseases and disorders such as hepatitis, rubella, influenza, rabies, hemorrhagic fever, lupus nephritis, and tick-borne encephalitis. The protein interacts in pathways such as NF-kappa signaling, cytosolic DNA-sensing, measles, influenza A, and mediated induction pathways with proteins such as CYLD, ZC3HAV1, DDX3X, MAVS, and WRNIP1.

Long Name

RIG-I

Alternate Names

DDX58, RIGI

Gene Symbol

RIGI

Additional RIG-I Products

Product Documents for RIG-I Antibody (Alme-1) - BSA Free

Certificate of Analysis

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Product Specific Notices for RIG-I Antibody (Alme-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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