Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RNF168 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human RNF168. The immunogen is located within amino acids 440 - 490 of RNF168. Amino Acid Squence: KEVDKEQMVPNRQKGSPD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%), Bovine (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNF168 Antibody - BSA Free

Western Blot: RNF168 AntibodyBSA Free [NBP1-76324]

Western Blot: RNF168 AntibodyBSA Free [NBP1-76324]

RNF168-Antibody-Western-Blot-NBP1-76324-img0002.jpg
Western Blot: RNF168 AntibodyBSA Free [NBP1-76324]

Western Blot: RNF168 AntibodyBSA Free [NBP1-76324]

Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] - RNF168 Antibody [NBP1-76324] - Human brain tissue lysate with RNF168 antibody at 1 ug/mL.
RNF168 Antibody - BSA Free

Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] -

Western Blot: RNF168 Antibody - BSA Free [NBP1-76324] - Ectopic expression of miR-15a, miR-16 sensitizes breast cancer cells to DNA damage through BMI1 dependent ubiquitination pathway. Western blot analysis showing the expression of BMI1, RING1A, RING1B, EZH2, gamma -H2AX, Ub-H2A, p-CHK2, p-ATM, RNF8, RNF168, MEL18, p53BP, BRCA1 proteins in MDAMB-231 cells transfected with miR-15a, miR-16 or both miR-15a/16 under etoposide-induced DNA damage conditions. Actin served as a gel loading control. Blots were cropped to enhance the representation (A,B). Immunofluorescence data showing accumulation of BMI1, ϒ-H2AX (C) RING1B (D) & Ub-H2A (E) p53BP (F) in MDAMB-231 cells transfected with miR-15a miR-16 or both miR-15a/16 under etoposide- treated conditions. Bar indicates 200 μm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28655885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RNF168 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNF168

RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.

Long Name

Ring Finger Protein 168

Alternate Names

E3 ubiquitin-protein ligase RNF168, EC 6.3.2, EC 6.3.2.-, FLJ39749, ring finger protein 168FLJ35794

Gene Symbol

RNF168

UniProt

NP_689830 (Human)

Additional RNF168 Products

Product Documents for RNF168 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RNF168 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
Epigenetics

Citations for RNF168 Antibody - BSA Free

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