RNF182 Antibody - BSA Free

Immunohistochemistry-Paraffin: RNF182 Antibody [NBP1-82707] -

Immunohistochemistry-Paraffin: RNF182 Antibody [NBP1-82707] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.

Immunohistochemistry-Paraffin: RNF182 Antibody [NBP1-82707] -

Immunohistochemistry-Paraffin: RNF182 Antibody [NBP1-82707] - Staining of human liver shows no positivity in hepatocytes as expected.

Western Blot: RNF182 Antibody - BSA Free [NBP1-82707] -

FR promotes the K48-linked ubiquitination of p65 by recruiting the E3 ubiquitin enzyme RNF182. Tenocytes were silenced with RNF182 siRNA (A), ING4 siRNA (B), or PPAR gamma siRNA (C) for 24 h and then treated with IL-1 beta (10 ng/mL) and FR (40 μM) for 24 h. Finally, the protein expression of p65 was detected by WB. (D) HEK293T cells were treated with RNF182 siRNA for 24 h, then transfected with HA-K48-Ub and Flag-p65 plasmids for 12 h, and next stimulated with 3-MA (5 mM) for 6 h, and finally treated with FR (40 μM) for 8 h. Cell lysates were collected for IP and WB detection. (E) Tenocytes were transfected with RNF182 siRNA for 24 h, then treated with 3-MA (5 mM) for 6 h, and finally treated with IL-1 beta (10 ng/mL) and FR (40 μM) for 24 h. Cell proteins were collected for IP. The expressions of p65 and K48-Ub in IP and WCL samples were detected by WB. (F) Flag-p65 and GFP-RNF182 plasmids were transfected into HEK293T cells for 24 h and then treated with FR (40 μM) for 8 h. Finally, WB was used to detect the protein of the Flag tag and GFP tag. (G) HEK293T cells were transfected with Flag-p65 and GFP-RNF182 plasmids for 24 h, then 3-MA (5 mM) was added for 6 h and then treated with FR (40 μM) for another 8 h. Finally, cell proteins were collected for IP, and proteins with Flag and GFP labels were detected by WB. (H) Tenocytes were pretreated with 3-MA (5 mM) for 6 h, followed by the addition of IL-1 beta (10 ng/mL) and FR (40 μM) for 24 h. Cell proteins were collected for IP. WB detected the protein expressions of p65 and RNF182 in IP and WCL samples. Data are expressed as the means +/- SD from three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35458235), licensed under a CC-BY license. Not internally tested by Novus Biologicals.