RNF2 Antibody (S04-2B3)
Novus Biologicals | Catalog # NBP3-14971
Recombinant Monoclonal Antibody
![Western Blot: RNF2 Antibody (S04-2B3) [NBP3-14971]](https://beta-resources.rndsystems.com/images/products/RNF2-Antibody-S04-2B3-Western-Blot-NBP3-14971-img0001.jpg "Western Blot: RNF2 Antibody (S04-2B3) [NBP3-14971]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S04-2B3 expressed in HEK293
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Product Specifications
Immunogen
A synthesized peptide derived from human RNF2 (Uniprot#: Q99496)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit RNF2 Antibody (S04-2B3) (NBP3-14971) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for RNF2 Antibody (S04-2B3)
Western Blot: RNF2 Antibody (S04-2B3) [NBP3-14971]
Western Blot: RNF2 Antibody (S04-2B3) [NBP3-14971] - Western blot detection of RNF2 in Hela, A549, HL-60, U2OS, U87-MG cell lysates using RNF2 Rabbit mAb (1:1000 diluted). Observed band size: 38kDa.![Immunohistochemistry: RNF2 Antibody (S04-2B3) [NBP3-14971]](https://beta-resources.rndsystems.com/images/products/RNF2-Antibody-S04-2B3-Immunohistochemistry-NBP3-14971-img0002.jpg "Immunohistochemistry: RNF2 Antibody (S04-2B3) [NBP3-14971]")
Immunohistochemistry: RNF2 Antibody (S04-2B3) [NBP3-14971]
Immunohistochemistry: RNF2 Antibody (S04-2B3) [NBP3-14971] - Immunohistochemical of RNF2 in Human lung cancer tissue using RNF2 antibody at dilution 1/50Applications for RNF2 Antibody (S04-2B3)
Application
Recommended Usage
Immunohistochemistry
1/50-1/200
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1/500-1/2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RNF2
Long Name
Ring Finger Protein 2
Alternate Names
DING, HIPI3, RING1B, RING2
Gene Symbol
RNF2
Additional RNF2 Products
Product Documents for RNF2 Antibody (S04-2B3)
Product Specific Notices for RNF2 Antibody (S04-2B3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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