RNMT [p Tyr1003] Antibody

Novus Biologicals | Catalog # NBP2-29681

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RNMT Primary Antibodies »

Product Specifications

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-Met (Tyr1003).

Reactivity Notes

Human

Modification

p Tyr1003

Specificity

Recognizes Met phosphorylated on Tyr1003.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

155 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Relevant Entrez Gene Numbers: NM_000245.2

Scientific Data Images for RNMT [p Tyr1003] Antibody

Western Blot: RNMT [p Tyr1003] Antibody [NBP2-29681]

Western Blot: RNMT [p Tyr1003] Antibody [NBP2-29681]

Western Blot: RNMT [p Tyr1003] Antibody [NBP2-29681] - Immunoblot Analysis Lysates from untreated (Lane 1) or HGF treated (Lanes 2 and 3) H69 cells were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-phospho-Met (Tyr1003) for 2 hours at room temperature. Lane 3 was pre-incubated with the blocking peptide immunogen corresponding to phospho-Met (Tyr1003). Proteins were visualized via HRP and chemiluminescent detection. Arrow indicates phospho-Met (Tyr1003)

Applications for RNMT [p Tyr1003] Antibody

Application
Recommended Usage

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+, pH 7.3), 1.0 mg/ml BSA (IgG, protease free) and 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNMT

mRNA cap methyltransferase (HCM) catalyzes the N7 methylation of the cap formed by mRNA capping enzyme (HCE) to form a terminal 7-methylguanosine. This is subsequently recognised by cap binding proteins that facilitate key events in gene expression.

Alternate Names

DKFZp686H1252, EC 2.1.1.56, hcm1p, hCMT1, hCMT1c, hMet, KIAA0398, mRNA (guanine-7-)methyltransferase, mRNA (guanine-N(7)-)-methyltransferase, mRNA cap guanine-N7 methyltransferase, mRNA cap methyltransferase, RG7MT1MET, RNA (guanine-7-) methyltransferase

Gene Symbol

RNMT

UniProt

P08581

Additional RNMT Products

Product Documents for RNMT [p Tyr1003] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RNMT [p Tyr1003] Antibody

Relevant Entrez Gene Numbers: NM_000245.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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