ROBO1 Antibody

Name: ROBO1 Antibody
Brand: Novus Biologicals
SKU: NBP3-23373
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23373

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

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Product Specifications

Immunogen

Robo1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human Robo1. This sequence has significant homology to the same region in rat and mouse Robo1, and has low homology to other Robo family members. [UniProt# Q9Y6N7]

Specificity

The antibody detects a 180 kDa band corresponding to Robo1 in Western blots of human HepG2 and HUVEC cells, as well as mouse C2C12 cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for ROBO1 Antibody

Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP3-23373] -

Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NBP3-23373] - Immunocytochemical labeling of ROBO1 in mouse C2C12. The cells were labeled with rabbit polyclonal ROBO1 (C-terminal region) in the absence or presence of blocking peptide. The antibody was then detected using appropriate secondary antibodies conjugated to Cy3.

Western Blot: ROBO1 Antibody [NBP3-23373] -

Western Blot: ROBO1 Antibody [NBP3-23373] - Western blot analysis of HepG2 (lanes 1 & 4), C2C12 (lanes 2 & 5), and HUVEC (lanes 3 & 6). The blot was probed with anti-ROBO1 (C-terminal region) in absence (lanes 1-3) or presence of ROBO1 (C-terminal region) blocking peptide (lanes 4-6).

Applications for ROBO1 Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: ROBO1

Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. In Drosophila, the roundabout gene, a member of the immunoglobulin gene superfamily, encodes an integral membrane protein that is both an axon guidance receptor and a cell adhesion receptor. This receptor is involved in the decision by axons to cross the central nervous system midline. The protein encoded by this gene is structurally similar to the Drosophila roundabout protein. Two transcript variants encoding different isoforms have been found for this gene.

Long Name

Roundabout Homolog 1

Alternate Names

DUTT1

Gene Symbol

ROBO1

Additional ROBO1 Products

Product Documents for ROBO1 Antibody

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Certificate of Analysis

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Product Specific Notices for ROBO1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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