ROR alpha/NR1F1 Antibody - BSA Free

Novus Biologicals | Catalog # NLS2337

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bat, Bovine, Canine, Equine, Hamster, Monkey, Primate, Rabbit

Predicted:

Chicken (95%), Fish (95%), Opossum (95%), Turkey (95%), Xenopus (95%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ROR alpha/NR1F1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 20 amino acid peptide from hinge domain of human ROR alpha/NR1F1.

Epitope

Hinge domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%).

Localization

Nuclear

Specificity

Human ROR alpha/NR1F1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RORB (65%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for ROR alpha/NR1F1 Antibody - BSA Free

Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody - BSA Free [NLS2337]

Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody - BSA Free [NLS2337]

Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody [NLS2337] - Human, Skin: Formalin-Fixed Paraffin-Embedded (FFPE)
Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody - BSA Free [NLS2337]

Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody - BSA Free [NLS2337]

Immunohistochemistry-Paraffin: ROR alpha/NR1F1 Antibody [NLS2337] - Human, Brain, Cortex: Formalin-Fixed Paraffin-Embedded (FFPE)

Applications for ROR alpha/NR1F1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

11 - 17 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: ROR alpha/NR1F1

Retinoid-related orphan receptor alpha (ROR alpha), a NR1 Thyroid Hormone-Like Receptor, has been shown to affect the development of the central nervous system. Deletion of ROR alpha in mice causes severe impairment in the differentiation of cerebellar Purkinje neurons and results in the "staggerer" phenotype. ROR alpha has been suggested as a potential target in the treatment of chronic inflammatory diseases, including atherosclerosis and rheumatoid arthritis. Four isoforms that differ in the N-terminal regions have been isolated, and their spatiotemporal expression appears to be under isoform-specific regulation. ROR alpha isoforms have the same DNA-binding domain but display different DNA binding specificities. ROR alpha 1 binds to and constitutively activates transcription from a large subset of ROR elements, while ROR alpha 2 recognizes ROR elements with strict specificity and displays weaker transcriptional activity. The N-terminal domain and zinc-finger region work in concert to confer high affinity and specific DNA-binding properties to the ROR isoforms and suggest a novel strategy for the control of DNA-binding activities of nuclear receptors. It has been suggested that melatonin may affect ROR alpha transcriptional activity and expression of ROR alpha-regulated genes via modulation of the Ca(2+)/CaM signaling pathway. The structure of the ligand-binding domain (LBD) of ROR alpha and a potential natural ligand have been elucidated recently (Kallen et al. 2002) ROR alpha expression has been documented in human adipose, brain, breast, heart. liver, lung, muscle, ovary, PBLs, prostate, small intestine, spleen and testis. ESTs have been isolated from cancerous human tissues including lung, tongue, skeletal muscle, and germ cell, and from human normal tissues including liver/spleen, testis, brain, and skin.

Long Name

Retinoic Acid-Related Orphan Receptor alpha

Alternate Names

NR1F1, RORA, RZRA

Entrez Gene IDs

6095 (Human)

Gene Symbol

RORA

Additional ROR alpha/NR1F1 Products

Product Documents for ROR alpha/NR1F1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ROR alpha/NR1F1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for ROR alpha/NR1F1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review ROR alpha/NR1F1 Antibody - BSA Free and earn rewards!

Have you used ROR alpha/NR1F1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for ROR alpha/NR1F1 Antibody - BSA Free (NLS2337):

Immunohistochemistry Protocol for ROR alpha Antibody (NLS2337):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies