ROR gamma/RORC/NR1F3 Antibody - BSA Free

Novus Biologicals | Catalog # NLS5188

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (93%), Monkey (93%), Orangutan (93%), Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ROR gamma/RORC/NR1F3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 14 amino acid peptide from modulating domain of human ROR gamma/RORC/NR1F3.

Epitope

Modulating domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Marmoset (86%), Mouse (86%), Rat (86%), Bat (86%), Bovine (86%).

Localization

Nuclear

Specificity

Human ROR gamma/RORC/NR1F3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NLS5188]

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NLS5188]

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NLS5188] - Analysis of anti-ROR Gamma antibody with human thymus.

Applications for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunohistochemistry-Paraffin

10 - 15 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: ROR gamma/RORC/NR1F3

ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma t, a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Alternate Names

NR1F3, RORC, RZRG

Entrez Gene IDs

6097 (Human)

Gene Symbol

RORC

UniProt

P51449

Additional ROR gamma/RORC/NR1F3 Products

Product Documents for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for ROR gamma/RORC/NR1F3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ROR gamma/RORC/NR1F3 Antibody - BSA Free

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Protocols

View specific protocols for ROR gamma/RORC/NR1F3 Antibody - BSA Free (NLS5188):

Immunohistochemistry Protocol for ROR gamma Antibody (NLS5188):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A synthetic sequence of 14 amino acids from modulating domain of Human ROR gamma.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A synthetic sequence of 14 amino acids from modulating domain of Human ROR gamma.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A synthetic sequence of 14 amino acids from modulating domain of Human ROR gamma.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

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