RPB8 Antibody (3G6-1A4) - Azide and BSA Free

Novus Biologicals | Catalog # H00005437-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3G6-1A4

Format

Azide and BSA Free
View all RPB8 Primary Antibodies »

Product Specifications

Immunogen

POLR2H (AAH00739, 1 a.a. ~ 150 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSRVYLLMKKLAF

Specificity

POLR2H - polymerase (RNA) II (DNA directed) polypeptide H

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RPB8 Antibody (3G6-1A4) - Azide and BSA Free

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01]

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01]

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - Analysis of POLR2H expression in transfected 293T cell line by POLR2H monoclonal antibody (M01), clone 3G6-1A4.Lane 1: POLR2H transfected lysate(17.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01]

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01]

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - POLR2H monoclonal antibody (M01), clone 3G6-1A4 Analysis of POLR2H expression in Hela.
Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01]

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01]

Western Blot: RPB8 Antibody (3G6-1A4) [H00005437-M01] - POLR2H monoclonal antibody (M01), clone 3G6-1A4. Analysis of POLR2H expression in HepG2.
Sandwich ELISA: RPB8 Antibody (3G6-1A4) [H00005437-M01] - Detection limit for recombinant GST tagged POLR2H is approximately 0.3ng/ml as a capture antibody.

Applications for RPB8 Antibody (3G6-1A4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RPB8

This gene encodes one of the essential subunits of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III.

Alternate Names

DNA-directed RNA polymerases I, II, and III subunit RPABC3, hsRPB8, II, and III 17.1 kDa polypeptide, II, and III subunit ABC3, polymerase (RNA) II (DNA directed) polypeptide H, RPB8 homolog

Entrez Gene IDs

5437 (Human)

Gene Symbol

POLR2H

OMIM

606023 (Human)

UniProt

AAH00739 (Human)

Additional RPB8 Products

Product Documents for RPB8 Antibody (3G6-1A4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RPB8 Antibody (3G6-1A4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RPB8 Antibody (3G6-1A4) - Azide and BSA Free

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