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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Insect, Zebrafish
Predicted:
Bovine (100%), Canine (100%), Chicken (98%), Porcine (99%), Rat (100%), Xenopus (96%), Zebrafish (98%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human RPL11. The exact sequence is proprietary.
Reactivity Notes
Xenopus laevis (96%), Drosophila (85%). Anopheles gambie (insect) reactivity reported by a customer review.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RPL11 Antibody
Western Blot: RPL11 Antibody [NBP2-20210]
Western Blot: RPL11 Antibody [NBP2-20210] - Binding to six Anopheles gambiae pupal lysis samples at 21.8 kDa, and the murine control at 20.6 kDa. Image from a verified customer review.Immunohistochemistry-Paraffin: RPL11 Antibody [NBP2-20210]
Immunohistochemistry-Paraffin: RPL11 Antibody [NBP2-20210] - RPL11 antibody detects RPL11 protein at cytosol and nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. RPL11 antibody dilution: 1:500.Western Blot: RPL11 Antibody [NBP2-20210]
Western Blot: RPL11 Antibody [NBP2-20210] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.Western Blot: RPL11 Antibody [NBP2-20210]
Western Blot: RPL11 Antibody [NBP2-20210] - Sample (50 ug of whole cell lysate) A: Mouse Liver, 12% SDS PAGE gel, diluted at 1:1000.Immunohistochemistry Whole Mount: RPL11 Antibody [NBP2-20210] -
Immunohistochemistry Whole Mount: RPL11 Antibody [NBP2-20210] -: RPL11 Antibody [NBP2-20210] - RPL11 antibody detects Rpl11 protein on whole-mount zebrafish embryos by immunohistochemical analysis.?Sample: Paraformaldehyde-fixed zebrafish embryos.
RPL11 antibody (NBP2-20210) dilution: 1:200.
Applications for RPL11 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:1000
Immunohistochemistry Whole-Mount
Assay dependent
Immunohistochemistry-Paraffin
1:100 - 1:1000
Western Blot
1:500 - 1:3000
Reviewed Applications
Read 1 review rated 5 using NBP2-20210 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RPL11
Alternate Names
cell growth-inhibiting protein 34,60S ribosomal protein L11, CLL-associated antigen KW-12, DBA7, GIG34, ribosomal protein L11
Gene Symbol
RPL11
UniProt
Additional RPL11 Products
Product Documents for RPL11 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RPL11 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for RPL11 Antibody (1)
5 out of 5
1 Customer Rating
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Customer Images
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Application: Western BlotSample Tested: Pupae cell lysis extracts and Pupal lysateSpecies: Anopheles gambie and Anopheles gambiaeVerified Customer | Posted 07/24/2019Binding to six Anopheles gambiae pupal lysis samples at 21.8 kDA, and the murine control at 20.6Bio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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