RRN3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-71822

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RRN3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 601 and 651 of human Transcription Initiation Factor IA using the numbering given in entry NP_060897.3 (GeneID 54700).

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RRN3 Antibody - BSA Free

Western Blot: RRN3 Antibody [NBP1-71822]

Western Blot: RRN3 Antibody [NBP1-71822]

Western Blot: RRN3 Antibody [NBP1-71822] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and Jurkat (J; 50 mcg) cells. Affinity purified rabbit anti-TIF-IA antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. TIF-IA was also immunoprecipitated by another rabbit anti-TIF-IA antibody which recognizes an upstream epitope.
Western Blot: RRN3 Antibody [NBP1-71822]

Western Blot: RRN3 Antibody [NBP1-71822]

Western Blot: RRN3 Antibody [NBP1-71822] - 20ug of WCE of 293T or NIH cells from a review by a confirmed customer.

Applications for RRN3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Reviewed Applications

Read 1 review rated 4 using NBP1-71822 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RRN3

RRN3 is required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent

Alternate Names

DKFZp566E104, RNA polymerase I-specific transcription initiation factor RRN3, RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae), RRN3 RNA polymerase I transcription factor homolog (yeast), TIF-IA, TIFIAMGC104238, Transcription initiation factor IA, transcription initiation factor TIF-IA

Gene Symbol

RRN3

UniProt

NP_060897.3 (Human)

Additional RRN3 Products

Product Documents for RRN3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RRN3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RRN3 Antibody - BSA Free (1)

4 out of 5
1 Customer Rating
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  • Name: Bryan Tinsley
    Application: Western Blot
    Sample Tested: 293T and NIH cells, transfected
    Species: Human
    Verified Customer | Posted 11/15/2013
    20 ug of whole cell extracts (SDS lysis buffer) from either 293T or NIH cells, transfected with mouse or human RRN3.
    RRN3 Antibody - BSA Free NBP1-71822

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