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Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser227 of human RSK2. The exact sequence is proprietary.
Modification
p Ser 227
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Centrifuge briefly prior to opening.
Scientific Data Images for RSK2 [p Ser227] Antibody
Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244]
Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244] - Untreated (-) and treated (+) A431 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK2 (phospho Ser227) antibody (NBP3-13244) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244]
Western Blot: RSK2 [p Ser227] Antibody [NBP3-13244] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK2 (phospho Ser227) antibody (NBP3-13244) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Applications for RSK2 [p Ser227] Antibody
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RSK2
Long Name
Ribosomal Protein S6 Kinase II
Alternate Names
ISPK-1, pp90RSK2, RPS6KA3
Gene Symbol
RPS6KA3
Additional RSK2 Products
Product Documents for RSK2 [p Ser227] Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for RSK2 [p Ser227] Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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