RUNX1/CBFA2 Antibody (6C12A5) - BSA Free

Novus Biologicals | Catalog # NBP2-61861

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6C12A5

Format

BSA Free
View all RUNX1/CBFA2 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RUNX1/CBFA2 (AA: 237-337) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

48.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUNX1/CBFA2 Antibody (6C12A5) - BSA Free

Western Blot: RUNX1/CBFA2 Antibody (6C12A5)BSA Free [NBP2-61861]

Western Blot: RUNX1/CBFA2 Antibody (6C12A5)BSA Free [NBP2-61861]

Western Blot: RUNX1/CBFA2 Antibody (6C12A5) [NBP2-61861] - Analysis using RUNX1 mAb against human RUNX1 (AA: 237-337) recombinant protein. (Expected MW is 36.9 kDa)
Flow Cytometry: RUNX1/CBFA2 Antibody (6C12A5) - BSA Free [NBP2-61861]

Flow Cytometry: RUNX1/CBFA2 Antibody (6C12A5) - BSA Free [NBP2-61861]

Flow Cytometry: RUNX1/CBFA2 Antibody (6C12A5) [NBP2-61861] - Analysis of K562 cells using RUNX1 mouse mAb (green) and negative control (red).
Western Blot: RUNX1/CBFA2 Antibody (6C12A5)BSA Free [NBP2-61861]

Western Blot: RUNX1/CBFA2 Antibody (6C12A5)BSA Free [NBP2-61861]

Western Blot: RUNX1/CBFA2 Antibody (6C12A5) [NBP2-61861] - Analysis using RUNX1 mAb against HEK293 (1) and RUNX1 (AA: 237-337)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: RUNX1/CBFA2 Antibody (6C12A5) - BSA Free [NBP2-61861]

ELISA: RUNX1/CBFA2 Antibody (6C12A5) - BSA Free [NBP2-61861]

ELISA: RUNX1/CBFA2 Antibody (6C12A5) [NBP2-61861] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for RUNX1/CBFA2 Antibody (6C12A5) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:200-1:400

Western Blot

1:500-1-2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUNX1/CBFA2

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Runt-related Transcription Factor 1

Alternate Names

AML1, CBFA2

Gene Symbol

RUNX1

Additional RUNX1/CBFA2 Products

Product Documents for RUNX1/CBFA2 Antibody (6C12A5) - BSA Free

Certificate of Analysis

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Product Specific Notices for RUNX1/CBFA2 Antibody (6C12A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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