RUNX3/CBFA3 Antibody (2B3) - BSA Free

Novus Biologicals | Catalog # NBP2-37455

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 2B3

Format

BSA Free
View all RUNX3/CBFA3 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RUNX3/CBFA3 (AA:186-252) expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

44.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUNX3/CBFA3 Antibody (2B3) - BSA Free

Western Blot: RUNX3/CBFA3 Antibody (2B3)BSA Free [NBP2-37455]

Western Blot: RUNX3/CBFA3 Antibody (2B3)BSA Free [NBP2-37455]

Western Blot: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Western blot analysis using RUNX3 mAb against HEK293 (1) and RUNX3 (AA: 186-252)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: RUNX3/CBFA3 Antibody (2B3) - BSA Free [NBP2-37455]

Immunohistochemistry: RUNX3/CBFA3 Antibody (2B3) - BSA Free [NBP2-37455]

Immunohistochemistry: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RUNX3 mouse mAb with DAB staining.
Flow Cytometry: RUNX3/CBFA3 Antibody (2B3) - BSA Free [NBP2-37455]

Flow Cytometry: RUNX3/CBFA3 Antibody (2B3) - BSA Free [NBP2-37455]

Flow Cytometry: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Flow cytometric analysis of NIH3T3 cells using RUNX3 mouse mAb (green) and negative control (red).
Western Blot: RUNX3/CBFA3 Antibody (2B3)BSA Free [NBP2-37455]

Western Blot: RUNX3/CBFA3 Antibody (2B3)BSA Free [NBP2-37455]

Western Blot: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Western blot analysis using RUNX3 mAb against human RUNX3 recombinant protein. (Expected MW is 33 kDa)
ELISA: RUNX3/CBFA3 Antibody (2B3) - BSA Free [NBP2-37455]

ELISA: RUNX3/CBFA3 Antibody (2B3) - BSA Free [NBP2-37455]

ELISA: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for RUNX3/CBFA3 Antibody (2B3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUNX3/CBFA3

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Long Name

Runt-related Transcription Factor 3

Alternate Names

AML2, CBFA3, PEBP2A3, PEBP2AC

Gene Symbol

RUNX3

Additional RUNX3/CBFA3 Products

Product Documents for RUNX3/CBFA3 Antibody (2B3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RUNX3/CBFA3 Antibody (2B3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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