Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 48M7F9
View all S100A8 Primary Antibodies »

Product Specifications

Immunogen

Within the range of amino acids 2-105 of human S100A8 were used as the immunogen for the antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for S100A8 Antibody (48M7F9)

Western Blot: S100A8 Antibody (48M7F9) [NBP2-25257]

Western Blot: S100A8 Antibody (48M7F9) [NBP2-25257]

Western Blot: S100A8/A9 Antibody (48M7F9) [NBP2-25257] - Analysis of S100A8 in A) recombinant protein, B) human spleen, and C) HL60 lysate using S1008A antibody at 0.5 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Paraffin: S100A8 Antibody (48M7F9) [NBP2-25257]

Immunohistochemistry-Paraffin: S100A8 Antibody (48M7F9) [NBP2-25257]

Immunohistochemistry-Paraffin: S100A8/A9 Antibody (48M7F9) [NBP2-25257] - FFPE human spleen stained with S100A8 antibody (2 ug/mL), peroxidase-conjugate and DAB chromogen. TMA was used for this test. Image using the Azide Free form of this antibody.

Applications for S100A8 Antibody (48M7F9)

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:100 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S100A8

S100A8 is a low-molecular weight member of the S100 family of calcium-binding protein which promotes tumorigenesis. It promotes cell migration and invasion through p38 MAP dependent NF-kappaB activation leading to an increase of MMP2 and MMP12 in gastric cancer (Kwon CH et al., 2013). The phagocyte-specific Ca2+-binding S100A8 protein has been proposed as an essential regulator of the plasma membrane NADPH oxidase activity. It is abundantly expressed in the cytosol of neutrophils and is able to form Ca2+-dependent heterocomplexes, with heterotetramers being a probable prerequisite for its biological activities in myeloid cells. S100A8 and S100A9 have been proposed as essential regulators that exert their role through interactions with NADPH oxidase subunits (Brechard S et al., 2013).S100A8 and S100A9 are generally considered proinflammatory. Whereas hypohalous acids generated by activated phagocytes promote novel modifications in murine S100A8 but modifications to human S100A8 are undefined and there is no evidence that these proteins scavenge oxidants in human disease. Oxidized S100A8 was prominent in lungs from patients with asthma and significantly elevated in sputum compared to controls. Results have broad implications for conditions under which hypohalous acid oxidants are generated by activated phagocytes. Identification in human disease of the novel S100A8 Cys derivatives typical of those generated in vitro strongly supports the notion that S100A8 contributes to antioxidant defense during oxidative stress (Gomes LH et al., 2013).PsA (Psoriatic arthritis) is a chronic inflammatory arthropathy associated with psoriasis. Histologically, PsA is characterized by lining layer hyperplasia, diffuse infiltrate of B, T, macrophages and dendritic cells associated with neutrophils' proliferation and angiogenesis. Histological findings are associated with monocyte-derived cytokines expression, as Myeloid-related protein (S100A8/A9). They play an important role in intracellular functions and cytoskeleton-membrane interactions. S100A8/A9 has a role in the propagation and perpetuation of the inflammatory process in patients with psoriasis and PsA, because of an activated monocyte/macrophage system that involve, distal to the skin, the "enthesal-complex." It also acts as one of the principal mediators of innate immune response, and has been proposed as diagnostic marker for several inflammatory conditions including neonatal sepsis. (Chimenti MS et al. 2013, Terrin G et al., 2012).

Long Name

S100 Calcium Binding Protein A8

Alternate Names

Calgranulin A, CFAG, MRP8

Gene Symbol

S100A8

UniProt

NP_038678 (Mouse)

Additional S100A8 Products

Product Documents for S100A8 Antibody (48M7F9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for S100A8 Antibody (48M7F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for S100A8 Antibody (48M7F9)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?

    A:

    Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

  • Q: We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.

    A:

    Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

  • Q: Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?

    A:

    Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.

  • Q: We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.

    A:

    Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

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