S1P2/EDG-5/S1PR2 Antibody (HL3765)
Novus Biologicals | Catalog # NBP3-48844
Recombinant Monoclonal Antibody
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL3765
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Product Specifications
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human S1P2/EDG-5/S1PR2. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for S1P2/EDG-5/S1PR2 Antibody (HL3765)
Western Blot: S1P2/EDG-5/S1PR2 Antibody (HL3765) [NBP3-48844] -
Unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.Western Blot: S1P2/EDG-5/S1PR2 Antibody (HL3765) [NBP3-48844] -
Unboiled various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:2400. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data are based on NCBI database.Western Blot: S1P2/EDG-5/S1PR2 Antibody (HL3765) [NBP3-48844] -
Boiled and unboiled HCT116 whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibodyWestern Blot: S1P2/EDG-5/S1PR2 Antibody (HL3765) [NBP3-48844] -
Unboiled various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with S1P2/EDG-5/S1PR2 antibody [HL3765] (NBP3-48844) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for S1P2/EDG-5/S1PR2 Antibody (HL3765)
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: S1P2/EDG-5
Long Name
Endothelial Differentiation Gene 5
Alternate Names
AGR16, EDG5, GPCR13, GPCR18, H218, LPB2, S1PR2
Gene Symbol
S1PR2
Additional S1P2/EDG-5 Products
Product Documents for S1P2/EDG-5/S1PR2 Antibody (HL3765)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for S1P2/EDG-5/S1PR2 Antibody (HL3765)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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