SAH3 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-400

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SAH3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human adenosylhomocysteinase 3 (KIAA0828) using the numbering given in entry NP_056143.1 (GeneID 23382).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAH3 Antibody - BSA Free

Western Blot: SAH3 Antibody [NB100-400]

Western Blot: SAH3 Antibody [NB100-400]

Western Blot: SAH3 Antibody [NB100-400] - Western blot and IP/WB using NB100-400. Hela whole cell lysates were probed with NB100-400 to detect endogenous SAH-3 or IPed with NB100-400 and detected with another rabbit polyclonal SAH-3 antibody.

Applications for SAH3 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:1000-1:10000
Application Notes
This antibody may be used for Western Blot, where a band should be seen at ~70/80 kDa. It may also be used for immunoprecipitation. *The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SAH3

In mammals, S-Adenosylhomocysteine hydrolase (SAH3 / AdoHcyase) catalyzes the hydrolysis of S-adenosylhomocysteine to form adenosine and homocysteine. AdoHcy is the product of all adenosylmethionine (AdoMet)-dependent biological transmethylations. These reactions have a wide range of products, and are common in all facets of biometabolism. As a product inhibitor, elevated levels of AdoHcy suppress AdoMet-dependent transmethylations. Thus, SAH3 is a regulator of biological transmethylation.

Alternate Names

adenosylhomocysteinase-like 2, AdoHcyase 3, ADOHCYASE3, EC 3.3.1.1, FLJ21719, KIAA0828S-adenosylhomocysteine hydrolase-like 2, putative adenosylhomocysteinase 3, S-adenosylhomocysteine hydrolase-like protein 2, S-adenosyl-L-homocysteine hydrolase 3

Entrez Gene IDs

23382 (Human)

Gene Symbol

AHCYL2

UniProt

Q96HN2 (Human)

Additional SAH3 Products

Product Documents for SAH3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SAH3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SAH3 Antibody - BSA Free

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