SAMM50 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-94852

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SAMM50 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SAMM50 (NP_056195.3). MGTVHARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTCQGDDALPNGLDVTFEVTELRRLTGSYNTMVGNNEGSMVLGLKLPNLLGRAEKVTFQFSYGTKETSYGLSFFKPRPGNFERNFSVNLYKVTGQFPWSSLRETDRGMSAEYSFPIW

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAMM50 Antibody - BSA Free

Western Blot: SAMM50 AntibodyBSA Free [NBP2-94852]

Western Blot: SAMM50 AntibodyBSA Free [NBP2-94852]

Western Blot: SAMM50 Antibody [NBP2-94852] - Analysis of extracts of various cell lines, using SAMM50 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP2-94852]

Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP2-94852]

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP2-94852] - Immunohistochemistry of paraffin-embedded mouse heart using SAMM50 Rabbit pAb (NBP2-94852) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP2-94852]

Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP2-94852]

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP2-94852] - Immunohistochemistry of paraffin-embedded rat heart using SAMM50 Rabbit pAb (NBP2-94852) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP2-94852]

Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP2-94852]

Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP2-94852] - Immunohistochemistry of paraffin-embedded human liver cancer using SAMM50 Rabbit pAb (NBP2-94852) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation: SAMM50 Antibody - BSA Free [NBP2-94852]

Immunoprecipitation: SAMM50 Antibody - BSA Free [NBP2-94852]

Immunoprecipitation: SAMM50 Antibody [NBP2-94852] - Immunoprecipitation analysis of 600ug extracts of Mouse heart cells using 3ug SAMM50 antibody (NBP2-94852). Western blot was performed from the immunoprecipitate using SAMM50 antibody (NBP2-94852) at a dilution of 1:1000.

Applications for SAMM50 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAMM50

SAMM50 is a component of the sorting and assembly machinery (SAM) complex of the outer mitochondrial membrane. The SAMcomplex has a role in integrating beta-barrel proteins into the outer mitochondrial membrane (Humphries et al., 2005(PubMed 15644312)).(supplied by OMIM)

Alternate Names

CGI-51, FLJ42905, FLJ99036, OMP85, SAM50, sorting and assembly machinery 50kDa, sorting and assembly machinery component 50 homolog, sorting and assembly machinery component 50 homolog (S. cerevisiae), TOB55, Transformation-related gene 3 protein, transformation-related protein 3, TRG-3FLJ35825, YNL026W

Gene Symbol

SAMM50

Additional SAMM50 Products

Product Documents for SAMM50 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SAMM50 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SAMM50 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review SAMM50 Antibody - BSA Free and earn rewards!

Have you used SAMM50 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies