SAP130 Antibody (23GB2260)

Novus Biologicals | Catalog # NBP3-50249

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2260
View all SAP130 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human SAP130 (Uniprot: Q15393)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAP130 Antibody (23GB2260)

SAP130 Antibody (23GB2260)

Immunocytochemistry/ Immunofluorescence: SAP130 Antibody (23GB2260)[NBP3-50249] -

Immunocytochemical staining of HepG2 cells with SAP130 antibody (1:1,000). Nuclei were stained blue with DAPI; SAP130 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
SAP130 Antibody (23GB2260)

Flow Cytometry: SAP130 Antibody (23GB2260)[NBP3-50249] -

Flow cytometric analysis of SAP130expression in HepG2 cells using SAP130 antibody (1:2,000). Green, isotype control; red, Splicing factor 3b subunit 3.
SAP130 Antibody (23GB2260)

Western Blot: SAP130 Antibody (23GB2260)[NBP3-50249] -

Western blotting analysis using SAP130 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for SAP130 Antibody (23GB2260)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAP130

SAP130 encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Alternate Names

KIAA0017splicing factor 3b, subunit 3, 130kD, Pre-mRNA-splicing factor SF3b 130 kDa subunit, RSE1, SAP130SAP 130, SF3B130, SF3b130pre-mRNA splicing factor SF3b, 130 kDa subunit, Spliceosome-associated protein 130, splicing factor 3B subunit 3, splicing factor 3b, subunit 3, 130kDa, STAF130

Gene Symbol

SF3B3

Additional SAP130 Products

Product Documents for SAP130 Antibody (23GB2260)

Certificate of Analysis

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Download SDS

Product Specific Notices for SAP130 Antibody (23GB2260)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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