SARDH Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93295

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all SARDH Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-560 of human SARDH (NP_009032.2). RIEGIQNMPNVRDHDASVYLRLQGDALSVGGYEANPIFWEEVSDKFAFGLFDLDWEVFTQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLGGGCGQELAHWIIHGRPEKDMHGYDIRRFHHSLTDHPRWIRERSHESYAKNYSVVFPHDEPLAGRNMRRDPLHEELLGQGCVFQERHGWERPGWFHPRGPAPVLEYDYYGAYGSRAHEDYAYRRLLADEYTFAFPPHHDTIKKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SARDH Antibody - Azide and BSA Free

SARDH Antibody - Azide and BSA Free

Western Blot: SARDH Antibody - Azide and BSA Free [NBP2-93295] -

Western Blot: SARDH Antibody - Azide and BSA Free [NBP2-93295] - Western blot analysis of various lysates, using SARDH Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 40s.
SARDH Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SARDH Antibody - Azide and BSA Free [NBP2-93295] -

Immunocytochemistry/ Immunofluorescence: SARDH Antibody - Azide and BSA Free [NBP2-93295] - Immunofluorescence analysis of L929 cells using SARDH Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for SARDH Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SARDH

Alternate Names

BPR-2, dimethylglycine dehydrogenase-like 1, DMGDHL1, FLJ36475, mitochondrial, SAR, sarcosine dehydrogenase, SARD, SDH

Gene Symbol

SARDH

Additional SARDH Products

Product Documents for SARDH Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SARDH Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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FAQs

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